XRCC6 Antibodies

X-ray repair cross-complementing protein 6 (XRCC6)

Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner.

It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6.

Gene Information

Human
Gene Name:	XRCC6
Uniprot:	P12956
Entrez:	2547

Family

Belongs to:
ku70 family

Alternative Names

5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; D22S731; EC 3.6.4.-; EC 4.2.99.-; G22P1; G22P1Ku70; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku70; ML8; ML8,70 kDa subunit; thyroid-lupus autoantigen p70; Thyroid-lupus autoantigen; TLAA; TLAAD22S671; X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6; X-ray repair cross-compleme

Molecular Weight

Mass (kDA):
69.843 kDA

Genomic Context

Human
Location:	22q13.2
Sequence:	22; NC_000022.11 (41621163..41664048)

Subcellular Localizations

Nucleus. Chromosome.

Diseases

• Malignant Neoplasms
• Carcinoma
• Malignant Neoplasm Of Breast
• Cholangiocarcinoma
• Neoplasms
• Genomic Instability
• Mammary Neoplasms
• Renal Cell Carcinoma
• Lymphoma
• Ovarian Neoplasm
• Liver Carcinoma
• Liver Neoplasms
• Genotoxic Stress

• Malignant Neoplasm Of Lung
• Malignant Neoplasm Of Ovary
• Cell Transformation, Neoplastic
• Lung Neoplasms
• Cystadenocarcinoma, Serous
• Lymphoproliferative Disorders
• Stomach Neoplasms

Interacting Proteins

• ARAP1
• BAX
• COIL
• DEAF1
• HMBOX1

• HTT
• NDRG1
• PPID
• PPM1G
• PRKDC

• SIRT1
• TCF7L2
• TERF2IP
• TP53
• TPT1

• WRN
• XRCC4
• XRCC5

Pathways

• Dna Repair
• Double-strand Break Repair
• V(d)j Recombination
• Drug Resistance
• Mismatch Repair
• Cell Cycle
• Pathogenesis
• Recombinational Repair
• Cell Proliferation
• G2 Phase
• Regulation Of Signaling
• Aging
• Immune Response

• Menopause
• Regulation Of Gene Expression
• Cell Cycle Arrest
• Reverse Transcription
• Cell Death
• Telomere Maintenance
• S Phase

PTMs

• Phosphorylation

• Cleavage

• Oxidation

Research Areas

• Breast Cancer
• Cancer
• DNA Double Strand Break Repair
• DNA Repair
• Non-homologous end-joining

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/XRCC6

Best Uses Of The XRCC6 Marker From Boster Bio

When it comes to using a gene as a marker there are a variety of ways to approach it. Boster Bio offers many products and services. This article will cover the XRCC6 Cell-Based ELISA Kit and the Anti-Glycophorin-A / CD235a Monoclonal Antibody. In addition, you'll be taught about the benefits of gene infographics and how to apply them to your research.

XRCC6 Cell Based ELISA Kit

The Ku70/XRCC6 cell-based ELSA kit is a simple and high-throughput method of monitoring Ku70/XRCC6's protein expression (Phosphoser5). This kit employs an indirect immunohistochemistry procedure to monitor the level of the protein target in cells. It is a fantastic choice for screening inhibitors and activators of the Ku70/XRCC6 protein.

Boster Bio has created several specific and sensitive antibodies that are highly sensitive. Boster Bio products are validated for IHC, WB, ELISA and Flow Cytometry. Their antibodies are cited in over 29,000 scientific publications. Boster Bio XRCC6 Cell-Based Kit utilizes antibodies that have been validated against human cancer cells as well as other samples.

Anti-Glycophorin a / CD235a Monoclonal Antibody

The Anti-Glycophorin A and CD235a Monoclonal Antibody from Boster Bio is an Mouse monoclonal antibody that is derived from GYPA, which is a recognized marker of erythrocytes. This antibody reacts with Human cells, allowing researchers to perform immunohistochemistry on these cells.

This antibody targets inflammatory disorders, such as atopic allergy as well as atopic dermatitis. It also targets atherosclerosis. It also has therapeutic applications in the field of immunology, tissue transplantation and infectious diseases. It is available in different concentrations and can be used for high- and low-level immunoreactivity in inflammatory conditions.

The molecule is made up of two polypeptide chains, one light and one heavy. Each chain is able to recognize the first antigen in presence of the second. Monoclonal antibodies are useful for clinical trials and cancer immunotherapy. It also recognizes two antigens, it's effective in cancer immunotherapy and recombinant DNA methods.

This antibody has a variety of distinct modes of action. Single-chain variable fragments (scFvs) are fusion proteins that contain variable domains in both the light and heavy chains, are called single-chain variable fragments. They join at the N-terminus of the VH and the C-terminus of the VL. A bispecific scFv is created by fusing two scFvs with different specificities.

The antibodies are created by transfection of CHO cells with three expression plasmids, one of which encodes the heavy chain and the third the light chain. The heavy chain antibodies produced by recombinant recombinant are then isolated using size exclusion chromatography and mass spectrometry. Boster scientists developed the pYACRC yeast synthetic chromosome virus, which is used to produce monoclonal antibodies.

The invention in question relates to production of heavy chain antibodies with CH2 domains that have been truncated. The FRs are responsible for maintaining the beta-sheet structure. These regions are responsible to the antigen-binding sites of the antibodies. However, these antibodies are also capable of recognizing and removing antigens that are present in bloodstream. Boster Bio's anti-GlycophorinA CD235a antibody has a high affinity to the CD235a protein. This protein is implicated in a myriad of illnesses.

Kit for ELISA

The XRCC6 ELISA Kit provides a quick and simple method to determine the levels in cultured cells of XRCC6. It can also be used to determine the effects of various inhibitors and treatments on XRCC6 protein expression. The ELISA Kit is stable at -20 or 4 degrees Celsius and can be stored for up to six months.

The XRCC6 ELISA Kit is based on Picokine(tm) platform which provides high-sensitivity in ELISAs. The Picokine(tm) technology enhances the ability of the ELISA kit to picogram levels. Boster Bio's Picoband technology is an ingenuous polymer-based secondary antibody which can save up to 30 minutes compared to traditional IHC. It is backed by technical support provided by the BeNeLux distributor Sanbio.

Gene infographics

The XRCC genes are a class of tumor-related genes which have been implicated in lung adenocarcinoma. These genes include XRCC1, XRCC2, XRCC6, FANCG and PRKDC. XRCC is an abbreviation for repair of cross-complementary X-rays. The acronym ROC refers to receiver operator characteristic, and AUC refers to the area under the curve. These genes have been associated with LUAD, a condition that is associated with a poorer prognosis.

Researchers have identified several immune factors that may be associated with the XRCC6 gene. XRCC6 is correlated with TNFRSF14, KLRK1, and BTLA. It has also been shown that it is linked to other genes that are related to tumors, like CD276, TNFSF13 and PDCD1LG2, and IL6R.

The Dmblm locus is home to the Bloom syndrome gene. To reverse the phenotypes of mutants the addition of a Ku70 copy is required. The Ku heterodimer is in dynamic equilibrium with Ku70/Ku80. XRCC4 is capable of interfacing directly with Ku70/Ku80. These proteins are necessary for the accumulation (194363) of XRCC4 at DNA double-strand break. Mari et. and (2006) suggested that XRCC4 functions as an elastic tether to Ku70/Ku80 as well as LIG4.