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Product Info Summary
| SKU: | LS003309 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5) |
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Product info
Product Name
GRP78 (HSPA5) (NM_005347) Human Over-expression Lysate
SKU/Catalog Number
LS003309
Size
100 µg
Description
Transient overexpression lysate of heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (HSPA5)
Storage & Handling
Cite This Product
GRP78 (HSPA5) (NM_005347) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003309)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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GRP78 (HSPA5) Human Over-expression Lysates NM_005347.
Western blot validation of overexpression lysate (LS003309) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with HSPA5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for HSPA5 (Source: Uniprot.org, NCBI)
Gene Name
HSPA5
Full Name
Endoplasmic reticulum chaperone BiP
Weight
72.333kDa
Superfamily
heat shock protein 70 family
Alternative Names
78 kDa glucose-regulated protein; BIP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; FLJ26106; GRP78; GRP-78; GRP78BIP; Heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSP70-5; HSPA5; Immunoglobulin heavy chain-binding protein; MIF2 HSPA5 BIP, GRP78, HEL-S-89n heat shock protein family A (Hsp70) member 5 endoplasmic reticulum chaperone BiP|78 kDa glucose-regulated protein|HSP70 family protein 5|binding-immunoglobulin protein|endoplasmic reticulum lumenal Ca(2+)-binding protein grp78|epididymis secretory sperm binding protein Li 89n|glucose-regulated protein, 78kDa|heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|heat shock protein 70 family protein 5|heat shock protein family A member 5|immunoglobulin heavy chain-binding protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on HSPA5, check out the HSPA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For GRP78 (HSPA5) (NM_005347) Human Over-expression Lysate (LS003309)
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