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Product Info Summary
| SKU: | FEK0960 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit
See all Angiopoietin-like Protein 4/ANGPTL4 products
SKU/Catalog Number
FEK0960
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0960)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L165-S406
Sensitivity
<300 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0960 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0960 is reactive to ANGPTL4 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0960 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angiopoietin-like Protein 4/ANGPTL4
Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0960-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0960-ST | Human Angiopoietin-like Protein 4/ANGPTL4 Standard | 2 | 40 ng/tube |
| FEK0960-A | Human ANGPTL4 Antibody A | 1 | 3ml |
| FEK0960-B | Human ANGPTL4 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D. | 0.013 | 0.159 | 0.252 | 0.395 | 0.620 | 0.991 | 1.502 | 2.064 |
Data Example Images
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Human ANGPTL4 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1210 | 4752 | 21031 | 1116 | 4712 | 21659 |
| Standard deviation | 81.07 | 261.36 | 967.43 | 81.47 | 325.13 | 974.66 |
| CV (%) | 6.7% | 5.5% | 4.6% | 7.3% | 6.9% | 4.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 4/ANGPTL4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1210 | 1128 | 1302 | 1147 | 1197 | 74.2 | 6.2% |
| Sample 2 | 4752 | 4862 | 5145 | 4510 | 4817 | 269.77 | 5.6% |
| Sample 3 | 21031 | 21450 | 20486 | 18843 | 20453 | 859.01 | 4.2% |
Protein Target Info & Infographic
Gene/Protein Information For ANGPTL4 (Source: Uniprot.Org, NCBI)
Gene Name
ANGPTL4
Full Name
Angiopoietin-related protein 4
Weight
45.214kDa
Alternative Names
Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein ANGPTL4 ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158 angiopoietin like 4 angiopoietin-related protein 4|PPARG angiopoietin related protein|fasting-induced adipose factor|hepatic angiopoietin-related protein|hepatic fibrinogen/angiopoietin-related protein|peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANGPTL4, check out the ANGPTL4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANGPTL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine® Quick ELISA Kit (FEK0960)
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