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Product Info Summary
| SKU: | EK1745 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Human Annexin A1 ELISA Kit PicoKine®
View all Annexin A1 ELISA kits
SKU/Catalog Number
EK1745
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Annexin A1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANXA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 100pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Annexin A1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1745)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A2-N346
Sensitivity
<100 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1745 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1745 is reactive to ANXA1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1745 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Annexin A1
ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca (2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1745-CAP | Anti-Human ANXA1 Pre-coated 96-well strip microplate | 1 |
| EK1745-ST | Human ANXA1 Standard | 2 vials, 10 ng/tube |
| EK1745-DA | Human ANXA1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.021 | 0.066 | 0.196 | 0.245 | 0.452 | 0.861 | 1.437 | 1.943 |
Data Example Images
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Human Annexin A1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Annexin A1, For serum, dilution ratio is 1:10 and concentration is around 20 ng/ml.
For plasma, dilution ratio is 1:500 and concentration is around 1 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Annexin A1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 299 | 1474 | 5209 | 303 | 1499 | 5311 |
| Standard deviation | 12.56 | 64.86 | 276.1 | 17.9 | 92.9 | 414.3 |
| CV (%) | 4.2% | 4.4% | 5.3% | 5.9% | 6.2% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Annexin A1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 299 | 282 | 293 | 299 | 293 | 6.94 | 2.3% |
| Sample 2 | 1474 | 1425 | 1389 | 1444 | 1433 | 30.83 | 2.1% |
| Sample 3 | 5209 | 4826 | 5016 | 4992 | 5010 | 135.84 | 2.7% |
Protein Target Info & Infographic
Gene/Protein Information For ANXA1 (Source: Uniprot.Org, NCBI)
Gene Name
ANXA1
Full Name
Annexin A1
Weight
38.714kDa
Superfamily
annexin family
Alternative Names
Annexin A1; annexin I (lipocortin I); Annexin I; Annexin-1; ANX1; ANXA1; Calpactin II; calpactin-2; chromobindin-9; Lipocortin I; LPC1; LPC1annexin-1; p35; Phospholipase A2 inhibitory protein ANXA1 ANX1, LPC1 annexin A1 annexin A1|annexin I (lipocortin I)|annexin-1|calpactin II|calpactin-2|chromobindin-9|epididymis secretory sperm binding protein|phospholipase A2 inhibitory protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANXA1, check out the ANXA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANXA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Annexin A1 ELISA Kit PicoKine® (EK1745)
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No publications found for EK1745
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Human Annexin A1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-08-17
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-08-17
Question
Q: Can Annexin A1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
A. Green
Verified customer
Asked: 2019-01-13
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-01-13
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-30
Question
Q: can I use citrate plasma as samples in Human Annexin A1 Picokine® ELISA Kit (Catalog # EK1745)?
H. Wu
Verified customer
Asked: 2018-03-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Annexin A1 causing degradation of its protein structure. Annexin A1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Annexin A1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-03-25
Question
Q: which procedure should I follow in order to thaw whole blood sample for Annexin A1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-05-01
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Annexin A1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-05-01
Question
Q: if the enzyme conjugated Annexin A1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Annexin A1 antigen?
T. King
Verified customer
Asked: 2015-12-24
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2015-12-24
