Product Info Summary
| SKU: | PROTQ04760 |
|---|---|
| Size: | Starting from 4ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human Glyoxalase I Recombinant Protein
View all GLO1 recombinant proteins
SKU/Catalog Number
PROTQ04760
Size
Starting from 4ug
Tag
His-Tag
Description
Human Glyoxalase I Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-184aa. Application(s): Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Cite This Product
Human Glyoxalase I Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ04760)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Predicted MW
20.8 kDa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Amino Acid Sequence
Human, Q04760, 1-184aa; ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHHBioactivity
Specific activity is > 400 units/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25°C.Assay dilution & Images
Validation Images & Assay Conditions
Click image to see more details
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For Human Glyoxalase I Recombinant Protein (PROTQ04760)
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