Human IL-1RA/IL1RN PicoKine™ Quick ELISA Kit

Human IL-1RA/IL1RN PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

Product Info Summary

SKU: FEK0782
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

Product Name

Human IL-1RA/IL1RN PicoKine™ Quick ELISA Kit

See all IL-1ra/IL-1F3/IL1RN products

SKU/Catalog Number

FEK0782

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-1RA/IL1RN PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-1RA/IL1RN PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0782)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R26-E177

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0782 is reactive to IL1RN in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0782 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK0782-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0782-STHuman IL-1ra/IL-1F3/IL1RN Standard 2 10 ng/tube
FEK0782-CHuman IL1RN Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0330.1240.1490.2530.4280.7601.4132.223

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5528485351305756
Standard deviation4.6220.1646.923.6724.7149.14
CV (%)8.4%7.1%5.5%7.2%8.1%6.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1ra/IL-1F3/IL1RN in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 155576151562.524.5%
Sample 22842862962952909.583.3%
Sample 385388483192487349.765.7%
*number of samples for each test n=16.

Gene/Protein Information For IL1RN (Source: Uniprot.Org, NCBI)

Uniprot ID

P18510

Gene ID

3557

Gene Name

IL1RN

Full Name

Interleukin-1 receptor antagonist protein

Weight

20.055kDa

Alternative Names

DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 IL1RN DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4 interleukin 1 receptor antagonist interleukin-1 receptor antagonist protein|IL1 inhibitor|intracellular IL-1 receptor antagonist type II|intracellular interleukin-1 receptor antagonist (icIL-1ra)|type II interleukin-1 receptor antagonist

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL1RN, check out the IL1RN Infographic

IL1RN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL1RN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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