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Product Info Summary
| SKU: | FEK0977 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit
See all C-Reactive Protein/CRP products
SKU/Catalog Number
FEK0977
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CRP / C Reactive Protein / PTX1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Crp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0977)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H20-S225
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0977 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0977 is reactive to CRP in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0977 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of C-Reactive Protein/CRP
C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0977-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0977-ST | Mouse C-Reactive Protein/CRP Standard | 2 | 10 ng/tube |
| FEK0977-A | Mouse CRP Antibody A | 1 | 3ml |
| FEK0977-B | Mouse CRP Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.035 | 0.071 | 0.139 | 0.260 | 0.384 | 0.666 | 1.120 | 1.877 |
Data Example Images
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Mouse CRP PicoKine Quick ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 254 | 1752 | 6770 | 265 | 1851 | 6930 |
| Standard deviation | 11.93 | 94.6 | 453.59 | 12.98 | 105.5 | 512.82 |
| CV (%) | 4.7% | 5.4% | 6.7% | 4.9% | 5.7% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of C-Reactive Protein/CRP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 254 | 243 | 280 | 249 | 256 | 13.82 | 5.4% |
| Sample 2 | 1752 | 1563 | 1416 | 1662 | 1598 | 97.47 | 6.1% |
| Sample 3 | 6770 | 5545 | 5924 | 6118 | 6089 | 395.78 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For CRP (Source: Uniprot.Org, NCBI)
Gene Name
CRP
Full Name
C-reactive protein
Weight
25.039kDa
Superfamily
pentraxin family
Alternative Names
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895 CRP PTX1 C-reactive protein C-reactive protein|C-reactive protein, pentraxin-related|pentraxin 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CRP, check out the CRP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CRP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CRP / C Reactive Protein / PTX1 PicoKine® Quick ELISA Kit (FEK0977)
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