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Product Info Summary
| SKU: | EK0895 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell lysates. |
Product info
Product Name
Human P53 ELISA Kit PicoKine®
SKU/Catalog Number
EK0895
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human P53 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TP53 in cell lysates. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human P53 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0895)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M1-D393
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0895 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0895 is reactive to TP53 in Human samples
Validated Sample Types
cell lysates.
Application Guarantee
EK0895 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of p53
P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0895-CAP | Anti-Human TP53 Pre-coated 96-well strip microplate | 1 |
| EK0895-ST | Human TP53 Standard | 2 vials, 10 ng/tube |
| EK0895-DA | Human TP53 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.027 | 0.108 | 0.172 | 0.296 | 0.536 | 1.062 | 1.525 | 2.013 |
Data Example Images
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Human P53 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human P53 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 275 | 1540 | 5055 | 302 | 1396 | 5437 |
| Standard deviation | 16.5 | 72.38 | 394.29 | 20.83 | 71.19 | 467.58 |
| CV (%) | 6% | 4.7% | 7.8% | 6.9% | 8.6% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of p53 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 275 | 249 | 256 | 233 | 253 | 15.07 | 5.9% |
| Sample 2 | 1540 | 1645 | 1702 | 1738 | 1656 | 74.86 | 4.5% |
| Sample 3 | 5055 | 4442 | 4533 | 4238 | 4567 | 301.31 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For TP53 (Source: Uniprot.Org, NCBI)
Gene Name
TP53
Full Name
Cellular tumor antigen p53
Weight
43.653kDa
Superfamily
p53 family
Alternative Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor antigen p53|antigen NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TP53, check out the TP53 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TP53: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human P53 ELISA Kit PicoKine® (EK0895)
Hello CJ!
EK0895 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of Heat Shock Protein 70 Gene and Its Correlation with Inflammatory Markers in Essential Hypertension
An innovative immunosensor for detection of tumor suppressor protein p53 in unprocessed human plasma and cancer cell lysates
CdS nanocrystals/graphene oxide-AuNPs based electrochemiluminescence immunosensor in sensitive quantification of a cancer biomarker: p53
CdS nanocrystals/graphene oxide-AuNPs based electrochemiluminescence immunosensor in sensitive quantification of a cancer biomarker: p53
Expression of Heat Shock Protein 70 Gene and Its Correlation with Inflammatory Markers in Essential Hypertension
Antitumor effects of crocin on human breast cancer cells
Hasanzadeh, M., Baghban, H.N., Mokhtarzadeh, A., Shadjou, N., & Mahboob S5. (2017). An innovative immunosensor for detection of tumor suppressor protein p53 in unprocessed human plasma and cancer cell lysates. International Journal of Biological M...
Sun Y, Yang J, Wang Lz, Sun Lr, Dong Q. Hum Exp Toxicol. 2014 Aug;33(8):855-62. Doi: 10.1177/0960327113511475. Epub 2013 Nov 25. Crocin Attenuates Cisplatin-Induced Liver Injury In The Mice.
Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.
Fu Z, Chen D, Cheng H, Wang F. Med Sci Monit. 2015 Jan 27;21:318-25. Doi: 10.12659/Msm.893265. Hypoxia-Inducible Factor-1?? Protects Cervical Carcinoma Cells From Apoptosis Induced By Radiation Via Modulation Of Vascular Endothelial Growth Factor ...
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human P53 ELISA Kit PicoKine®
Question
Q: how to analyze ELISA data? I have obtained p53 level in cell lysates.
Z. Williams
Verified customer
Asked: 2020-07-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-07-10
Question
Q: which procedure should I follow in order to thaw whole blood sample for p53 ELISA after freezing?
C. Martinez
Verified customer
Asked: 2019-02-13
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test p53 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-02-13
Question
I have used Western Blot to try to check the p53 expression from E. coli cells in which I have cloned a plasmid that contains the p53 gene. All I want to know is if my E. coli clones are making the p53 or not. However, I did not see any bands in WB. I want to try ELISA with the cell suspensions and check if the cell pellet and supernatant (after centrifugation) separately by ELISA would inform me whether p53 is intracellular or extracellular. What do you think about this approach?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
p53 is located in nucleus and cytoplasm mainly, and thus the p53 concentration in the supernatant may be low. Also, cell supernatant is not validated for our human p53 ELISA kit. The ELISA may not help a lot since it is negative in WB.
Boster Scientific Support
Answered: 2019-02-07
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of p53 in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-05-06
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the p53 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-06
Question
Q: Can p53 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
A. Kumar
Verified customer
Asked: 2018-03-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-03-01
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement p53 using the EK0895 Human p53 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-01-13
Question
Q: if the enzyme conjugated p53 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the p53 antigen?
Verified Customer
Verified customer
Asked: 2017-08-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-08-06
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-11-23
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-11-23
Question
Q: What is the optimal O.D. value for p53 ELISA kit? I performed your p53 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain p53 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-05-01
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this p53 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of p53 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-05-01
