Click image to see more details
Product Info Summary
| SKU: | EK1449 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human PD-L1/B7-H1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1449
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human PD-L1/B7-H1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD274 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human PD-L1/B7-H1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1449)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: F19-R238
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1449 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1449 is reactive to CD274 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1449 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PD-L1
Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1449-CAP | Anti-Human CD274 Pre-coated 96-well strip microplate | 1 |
| EK1449-ST | Human CD274 Standard | 2 vials, 10 ng/tube |
| EK1449-DA | Human CD274 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.035 | 0.083 | 0.160 | 0.251 | 0.432 | 0.663 | 1.289 | 1.839 |
Data Example Images
Click image to see more details
Human PD-L1/B7-H1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PD-L1, Dilution ratio of 1:1, concentration in serum and plasma is 35-127 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PD-L1/B7-H1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 168 | 556 | 1564 | 151 | 519 | 1408 |
| Standard deviation | 6.83 | 23.35 | 79.76 | 10.26 | 22.31 | 97.15 |
| CV (%) | 4.9% | 4.2% | 6.9% | 6.8% | 4.3% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PD-L1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 168 | 168 | 181 | 182 | 174 | 6.75 | 3.8% |
| Sample 2 | 556 | 591 | 560 | 586 | 573 | 15.41 | 2.6% |
| Sample 3 | 1564 | 1419 | 1348 | 1388 | 1429 | 81.49 | 5.7% |
Protein Target Info & Infographic
Gene/Protein Information For CD274 (Source: Uniprot.Org, NCBI)
Gene Name
CD274
Full Name
Programmed cell death 1 ligand 1
Weight
33.275kDa
Superfamily
immunoglobulin superfamily
Alternative Names
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1 CD274 B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 CD274 molecule programmed cell death 1 ligand 1|B7 homolog 1|CD274 antigen|PDCD1 ligand 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD274, check out the CD274 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD274: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human PD-L1/B7-H1 ELISA Kit PicoKine® (EK1449)
Hello CJ!
EK1449 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
PD-L1 Ameliorates Murine Acute Graft-Versus-Host Disease by Suppressing Effector But Not Regulatory T Cells Function
PD-L1 Ameliorates Murine Acute Graft-Versus-Host Disease by Suppressing Effector But Not Regulatory T Cells Function
Liu ZH, Zheng FF, Mao YL, Ye LF, Bian J, Lai DH, Ye YL, Dai YP. Oncol Lett. 2017 Jun;13(6):4818-4824. doi: 10.3892/ol.2017.6080. Epub 2017 Apr 24. Effects of programmed death-ligand 1 expression on OK-432 immunotherapy following transurethral rese...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human PD-L1/B7-H1 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Human PD-L1/B7-H1 ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
14 Customer Q&As for Human PD-L1/B7-H1 ELISA Kit PicoKine®
Question
Is it possible to use serum as a test sample for EK1449?
Verified customer
Asked: 2021-01-29
Answer
Our lab tested serum collected from healthy human using the Human PD-L1/B7-H1 ELISA Kit PicoKine™ (EK1449). The concentration range of tested serum sample is 30-120pg/ml. Please see COA of the most current lot.
Boster Scientific Support
Answered: 2021-01-31
Question
Q: What is the optimal O.D. value for PD-L1 ELISA kit? I performed your PD-L1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain PD-L1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-10-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PD-L1 ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of PD-L1 that can be declared/interpreted as positive by the above standard is 12pg/ml.
Boster Scientific Support
Answered: 2020-10-18
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-06-02
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-06-02
Question
Q: which procedure should I follow in order to thaw whole blood sample for PD-L1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-12-29
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PD-L1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-12-29
Question
Does EK1449 recognize soluble PDL2?
Verified customer
Asked: 2019-09-20
Answer
The Human PD-L1/B7-H1 ELISA Kit PicoKine™ (EK1449) recognizes soluble PDL1. The immunogen sequence for the standard, detection antibody, and capture antibody is F19-R238 (uniprot: Q9NZQ7).
Boster Scientific Support
Answered: 2019-09-22
Question
Is it possible to extract exosome from samples and detect PD-L1 from the exosome extraction to verify if EK1450 and EK1449 indeed detect exosomal PD-L1?
Verified customer
Asked: 2019-04-29
Answer
Our lab isn't able to perform the experiment of extracting exosome from samples and detecting PD-L1 from the exosome extraction.
Boster Scientific Support
Answered: 2019-04-30
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of PD-L1 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PD-L1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-01-23
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis PD-L1 using the EK1449 Human PD-L1 Picokine® ELISA Kit?
Y. Wright
Verified customer
Asked: 2018-06-15
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-06-15
Question
Q: can I use citrate plasma as samples in Human PD-L1 Picokine® ELISA Kit (Catalog # EK1449)?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of PD-L1 causing disruption of its protein structure. PD-L1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the PD-L1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-02-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2017-04-19
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-04-19
Question
Q: Can PD-L1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2016-09-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-09-29
Question
I am interested in your PD-L1 ELISA kit, EK1449 and EK1450. May I know if these react to soluble and exosomal membrane form PD-L1? And may I know if you have done such testing to confirm it?
Verified Customer
Verified customer
Asked: 2015-02-02
Answer
Our lab technicians worked on lysates from human PBMCs and conditioned media from unstimulated human PBMCs using EK1449 and EK1450. The concentration of PD-L1 in human PBMCs lysate is around 600pg/mL-700pg/mL while that of PD-L1 in the culture media is around 80-100pg/mL. Our lab technicians thought that EK1449 and EK1450 are able to detect total PD-L1 in samples.
Boster Scientific Support
Answered: 2015-02-02
Question
Q: how to analyze ELISA data? I have performed PD-L1 level in serum.
L. Garcia
Verified customer
Asked: 2014-10-29
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-10-29
Question
Can RIPA with 1% SDS and 1% triton X-100 could be used for protein collection for cell lysate sample preparation?
Verified Customer
Verified customer
Asked: 2013-09-20
Answer
It is not recommended to use with 1% SDS and 1% triton X-100 for protein collection for cell lysate sample preparation.The concentration of 1% SDS is high, which might affect the sample preparation. <br> The customer can use Boster RIPA lysis buffer (SKU AR0105) or ultrasonicate the sample in 0.01M PBS. https://www.bosterbio.com/ripa-lysis-buffer-ar0105-
Boster Scientific Support
Answered: 2013-09-20
