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Product Info Summary
| SKU: | EK0581 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Resistin ELISA Kit PicoKine®
SKU/Catalog Number
EK0581
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Resistin ELISA Kit PicoKine™ (96 Tests). Quantitate Human RETN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Resistin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0581)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: K19-P108
Sensitivity
<3 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0581 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0581 is reactive to RETN in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0581 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Resistin
Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0581-CAP | Anti-Human RETN Pre-coated 96-well strip microplate | 1 |
| EK0581-ST | Human RETN Standard | 2 vials, 10 ng/tube |
| EK0581-DA | Human RETN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.000 | 0.121 | 0.208 | 0.367 | 0.660 | 0.989 | 1.452 | 1.990 |
Data Example Images
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Human Resistin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Resistin, Dilution ratio of 1:10, concentration in serum and plasma is around 20 ng/ml..
Some articles we found to cite concentrations of Resistin in samples: 23400230 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Resistin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 135 | 639 | 2067 | 142 | 636 | 2227 |
| Standard deviation | 8.1 | 40.25 | 161.22 | 8.52 | 46.42 | 177.83 |
| CV (%) | 6% | 7.3% | 7.8% | 6% | 7.3% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Resistin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 135 | 137 | 143 | 146 | 140 | 4.43 | 3.1% |
| Sample 2 | 639 | 653 | 634 | 737 | 665 | 41.72 | 6.2% |
| Sample 3 | 2067 | 2270 | 2337 | 2337 | 2252 | 110.67 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For RETN (Source: Uniprot.Org, NCBI)
Gene Name
RETN
Full Name
Resistin
Weight
11.419kDa
Superfamily
resistin/FIZZ family
Alternative Names
Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1 RETN ADSF, FIZZ31, RSTN, XCP1, RETN resistin resistin|C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1|adipose tissue-specific secretory factor|c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein|cysteine-rich secreted protein A12-alpha-like 2|cysteine-rich secreted protein FIZZ3|found in inflammatory zone 3|resistin delta2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RETN, check out the RETN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RETN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Resistin ELISA Kit PicoKine® (EK0581)
Hello CJ!
EK0581 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease
Adipocytokine concentrations in children with different types of beta-thalassemia
Resistin as a Biomarker for the Prediction of Left Atrial Substrate and Recurrence in Patients with Drug-Refractory Atrial Fibrillation Undergoing Catheter Ablation
Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study
Oxidative stress and adipokine levels were significantly correlated in diabetic patients with hyperglycemic crises
Glucose and lipid-related biomarkers and the antidepressant response to infliximab in patients with treatment-resistant depression
Protein and gene markers of metabolic dysfunction and inflammation together associate with functional connectivity in reward and motor circuits in depression
Adipocytokine concentrations in children with different types of beta-thalassemia
Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease
Niu XH, Li L, Li JY, Song Q, Jin MM, Liu JX. Medicine (Baltimore). 2017 Apr;96(16):e6622. doi: 10.1097/MD.0000000000006622. Serum resistin positively correlates with serum lipids, but not with insulin resistance, in first-degree relatives of type-...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Human Resistin ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for Resistin ELISA after freezing?
R. Edwards
Verified customer
Asked: 2020-09-10
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Resistin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-09-10
Question
Q: can you suggest the dilution ratio of serum samples for detection of Resistin in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
H. Anand
Verified customer
Asked: 2020-08-12
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Resistin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-08-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
A. Rodriguez
Verified customer
Asked: 2020-08-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-03
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Wilson
Verified customer
Asked: 2020-08-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-08-02
Question
Q: how to proceed with the analysis of ELISA data? I measured Resistin level in plasma.
M. Jha
Verified customer
Asked: 2020-03-04
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-03-04
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-08-22
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Resistin using the EK0581 Human Resistin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-12-30
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-12-30
Question
Q: is it okay to use citrate plasma as samples in Human Resistin Picokine® ELISA Kit (Catalog # EK0581)?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Resistin causing degradation of its protein structure. Resistin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Resistin ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-11-07
Question
What is the reference range of this kit in human serum?
Verified Customer
Verified customer
Asked: 2014-03-28
Answer
The reference range of this kit in human serum is 5-40ng/mL.
Boster Scientific Support
Answered: 2014-03-28
