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Product Info Summary
| SKU: | EK0513 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (EDTA) and urine. |
Product info
Product Name
Human TGF Beta 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0513
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TGF Beta 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TGFB1 in cell culture supernatants, serum, plasma s (EDTA) and urine. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TGF Beta 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0513)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: A279-S390
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0513 for more details
Cross-reactivity
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Reactive Species
EK0513 is reactive to TGFB1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (EDTA) and urine.
Application Guarantee
EK0513 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGFB1
Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0513-CAP | Anti-Human TGFB1 Pre-coated 96-well strip microplate | 1 |
| EK0513-ST | Human TGFB1 Standard | 2 vials, 10 ng/tube |
| EK0513-DA | Human TGFB1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.044 | 0.184 | 0.243 | 0.419 | 0.819 | 1.163 | 1.712 | 2.022 |
Data Example Images
Click image to see more details
Human TGF beta 1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TGFB1, For serum, dilution ratio is 1:200 and concentration is 14631-76099 pg/ml.
For plasma, dilution ratio is 1:200 and concentration is 722-1984 pg/ml..
Some articles we found to cite concentrations of TGFB1 in samples: 21786325 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TGF Beta 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 30 | 156 | 497 | 28 | 164 | 537 |
| Standard deviation | 1.38 | 8.42 | 34.79 | 1.73 | 8.85 | 38.12 |
| CV (%) | 4.6% | 5.4% | 7% | 6.2% | 5.4% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TGFB1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 30 | 29 | 32 | 28 | 29 | 1.47 | 5% |
| Sample 2 | 156 | 166 | 150 | 158 | 157 | 5.72 | 3.6% |
| Sample 3 | 497 | 448 | 471 | 476 | 473 | 17.42 | 3.6% |
Protein Target Info & Infographic
Gene/Protein Information For TGFB1 (Source: Uniprot.Org, NCBI)
Gene Name
TGFB1
Full Name
Transforming growth factor beta-1 proprotein
Weight
44.341kDa
Superfamily
TGF-beta family
Alternative Names
CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFB1, check out the TGFB1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TGF Beta 1 ELISA Kit PicoKine® (EK0513)
Hello CJ!
EK0513 has been cited in 105 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The role of Nrf2/PIWIL2/purine metabolism axis in controlling radiation-induced lung fibrosis
Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage
HSV-1 infection suppresses TGF-β1 and SMAD3 expression in human corneal epithelial cells
MiR-663 inhibits radiation-induced bystander effects by targeting TGFB1 in a feedback mode
Activation of calcium‑sensing receptor‑mediated autophagy in high glucose‑induced cardiac fibrosis in vitro
Calcium‑sensing receptor promotes high glucose‑induced myocardial fibrosis via upregulation of the TGF‑β1/Smads pathway in cardiac fibroblasts
Pigment epithelium‑derived factor protects human glomerular mesangial cells from diabetes via NOXO1‑iNOS suppression
Clinical significance of serum transforming growth factor‑β1 and procollagen type I N‑propeptide in post‑tuberculosis tracheobronchial stenosis
Thalidomide decreases high glucose‑induced extracellular matrix protein synthesis in mesangial cells via the AMPK pathway
Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Human TGF Beta 1 ELISA Kit PicoKine®
Question
What is the suggested dilution factor serum and plasma samples using EK0513?
Verified customer
Asked: 2021-02-17
Answer
For the Human TGF Beta 1 ELISA Kit PicoKine™ (EK0513), please see the following suggested dilution factors. Serum sample 1:100 Plasma sample 1:5 We suggest to run a pilot test to figure out the optimal dilution factor for their specific sample types.
Boster Scientific Support
Answered: 2021-02-20
Question
Is EK0513 specific to active protein only?
Verified customer
Asked: 2021-01-21
Answer
Yes, the Human TGF Beta 1 ELISA Kit PicoKine™ (EK0513) is specific to active protein only. To detect TGFB1 in samples, TGFB1 in samples needs to be activated prior to the assay.
Boster Scientific Support
Answered: 2021-01-22
Question
Is EK0513 specific for active protein only?
Verified customer
Asked: 2021-01-21
Answer
Yes, the Human TGF Beta 1 ELISA Kit PicoKine™ (EK0513) is specific to active protein only. To detect TGFB1 in samples, TGFB1 in samples needs to be activated prior to the assay.
Boster Scientific Support
Answered: 2021-01-22
Question
Q: if the enzyme conjugated TGFB1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGFB1 antigen?
Verified Customer
Verified customer
Asked: 2020-10-24
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-10-24
Question
Q: can you recommend the dilution ratio of serum samples for detection of TGFB1 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGFB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-02-07
Question
One of our customers used the Human TGF Beta 1 PicoKine
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Boster and Peprotech might apply different process and method to manufacuture the proteins, which would cause the kit unable to detect cat# 100-21C. EK1513 is able to detect recombinant proteins from R&D systems.
Boster Scientific Support
Answered: 2020-01-08
Question
Will EK0513 work with cynomolgus monkey?
Verified customer
Asked: 2019-11-08
Answer
Our lab hasn't woked on samples from cynomolgus monkey using Human TGF Beta 1 ELISA Kit PicoKine EK0513. The immunogen sequence of the antibody pairs as well as the standards is A279-S390. The sequence homology with cynomolgus monkey is 100%. It is suggested to run pilot tests with samples from cynomolgus monkey.
Boster Scientific Support
Answered: 2019-11-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement TGFB1 using the EK0513 Human TGFB1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-05-20
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Walker
Verified customer
Asked: 2019-04-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-04-09
Question
Q: What is the optimal O.D. value for TGFB1 ELISA kit? I performed your TGFB1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TGFB1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-03-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGFB1 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TGFB1 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-03-02
Question
Q: Can TGFB1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-10-13
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-10-13
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Anderson
Verified customer
Asked: 2018-02-15
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-02-15
Question
We have purchased your EK0513 kit for our experiments and we noticed on the datasheet that there was a need for activation of the TGFb prior to ELISA. Could you explain why this step as necessary?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
EK0513 is used for the quantitative determination of activated human TGF Beta 1 concentrations in cell culture supernates, serum, plasma(EDTA), and urine. Because of this, it is a mandatory step to activate the TGFb prior to ELISA.
Boster Scientific Support
Answered: 2017-12-12
Question
I would like to purchase the Human TGF Beta 1 ELISA Kit PicoKine to measure TGF concentration in human serum samples. Could you please provide me some tips on sample preparation?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
To condition a medium it is better to prepare serum free or low serum medium because sera contains cytokines. Day 0 seed 1M cells in 100mm tissue culture plate with complete medium. Day 3, remove the medium and replace it with 6-8 ml of low serum medium. Day 5 collect the medium into 15 ml tube. Spin at 2,000 rpm in centrifuge at 4 C for 10 minutes. Then save the supernatant and transfer it into 1.5 ml Eppendorf tubes. Store the supernatant at -80oC until experiment.
Boster Scientific Support
Answered: 2017-10-25
Question
Q: how to analyze ELISA data? I have obtained TGFB1 level in serum.
B. Surve
Verified customer
Asked: 2017-09-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-09-18
