Product Info Summary
SKU: | EK0519 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human TIE2/TEK ELISA Kit PicoKine®
SKU/Catalog Number
EK0519
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TIE2 ELISA Kit PicoKine® (96 Tests). Quantitate Human TEK in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TIE2/TEK ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0519)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A23-K745
Sensitivity
<5 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0519 for more details
Cross-reactivity
There is cross-reactivity with human TIE1< 0.1%.
Reactive Species
EK0519 is reactive to TEK in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0519 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Tie-2
Tyrosine kinase with Ig and EGF homology domain 2 (Tie-2), also called TEK tyrosine kinase, endothelial (TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK0519-CAP | Anti-Human TEK Pre-coated 96-well strip microplate | 1 |
EK0519-ST | Human TEK Standard | 2 vials, 10 ng/tube |
EK0519-DA | Human TEK Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.021 | 0.075 | 0.120 | 0.231 | 0.430 | 0.888 | 1.623 | 2.071 |
Data Example Images
Click image to see more details
Human TIE2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Tie-2, Dilution ratio of 1:10, concentration in serum and plasma is 14-90 ng/ml..
Some articles we found to cite concentrations of Tie-2 in samples: 24254563 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TIE2/TEK ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 305 | 1860 | 4704 | 311 | 1714 | 4949 |
Standard deviation | 23.79 | 81.84 | 263.42 | 27.05 | 99.41 | 351.37 |
CV (%) | 7.8% | 4.4% | 5.6% | 8.7% | 5.8% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Tie-2 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 305 | 289 | 298 | 271 | 290 | 12.73 | 4.3% |
Sample 2 | 1860 | 1842 | 2015 | 1990 | 1926 | 76.52 | 3.9% |
Sample 3 | 4704 | 5338 | 5060 | 5168 | 5067 | 232.08 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For TEK (Source: Uniprot.Org, NCBI)
Gene Name
TEK
Full Name
Angiopoietin-1 receptor
Weight
125830 MW
Superfamily
protein kinase superfamily
Alternative Names
Angiopoietin-1 receptor;2.7.10.1;Endothelial tyrosine kinase;Tunica interna endothelial cell kinase;Tyrosine kinase with Ig and EGF homology domains-2;Tyrosine-protein kinase receptor TEK;Tyrosine-protein kinase receptor TIE-2;hTIE2;p140 TEK;CD202b;TEK;TIE2, VMCM, VMCM1; TEK CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1 TEK receptor tyrosine kinase angiopoietin-1 receptor|TEK tyrosine kinase, endothelial|endothelial tyrosine kinase|tunica interna endothelial cell kinase|tyrosine kinase with Ig and EGF homology domains-2|tyrosine-protein kinase receptor TEK|tyrosine-protein kinase receptor TIE-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TEK, check out the TEK Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TEK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TIE2/TEK ELISA Kit PicoKine® (EK0519)
Hello CJ!
EK0519 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with proliferative diabetic retinopathy
Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with proliferative diabetic retinopathy
Sancakdar E, Guven As, Uysal Eb, Deveci K, G??lt??rk E. Pediatr Infect Dis J. 2015 Aug;34(8):E200-5. Doi: 10.1097/Inf.0000000000000706. Important Of Angiopoietic System In Evaluation Of Endothelial Damage In Children With Crimean-Congo Hemorrhagic...
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Customer Q&As
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4 Customer Q&As for Human TIE2/TEK ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated Tie-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Tie-2 antigen?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-02-03
Question
Q: which procedure should I follow in order to thaw whole blood sample for Tie-2 ELISA after freezing?
K. Li
Verified customer
Asked: 2020-01-06
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Tie-2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-01-06
Question
Q: how do I analyze ELISA data? I measured Tie-2 level in cell lysates.
Verified Customer
Verified customer
Asked: 2019-10-12
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-10-12
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-09-18