Product Info Summary
SKU: | PROTP18430 |
---|---|
Size: | 2ug, 10ug, 0.1mg |
Origin Species: | Pig |
Source: | Escherichia coli |
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Product info
Product Name
IL-1-alpha Interleukin-1 alpha Porcine Recombinant Protein
View all IL-1 alpha/IL-1F1 recombinant proteins
SKU/Catalog Number
PROTP18430
Size
2ug, 10ug, 0.1mg
Description
Interleukin-1A Porcine Recombinant produced in E. coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton. The IL-1A is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Cite This Product
IL-1-alpha Interleukin-1 alpha Porcine Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP18430)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Predicted MW
30.607kDa
Reconstitution
It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Thr-Tyr-Ser.
Biological Activity
The ED50 as determined by the dose-dependant stimulation of D10S cells is less than 0.03 ng/ml.
Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
Click image to see more details
Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For IL1A (Source: Uniprot.org, NCBI)
Gene Name
IL1A
Full Name
Interleukin-1 alpha
Weight
30.607kDa
Superfamily
IL-1 family
Alternative Names
Hematopoietin-1; Lymphocyte-activating factor (LAF); Endogenous Pyrogen (EP); Leukocyte Endogenous Mediator (LEM); Mononuclear Cell Factor (MCF); IL-1 alpha; IL1; IL-1A; IL1F1 IL1A IL-1 alpha, IL-1A, IL1, IL1-ALPHA, IL1F1 interleukin 1 alpha interleukin-1 alpha|hematopoietin-1|preinterleukin 1 alpha|pro-interleukin-1-alpha
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL1A, check out the IL1A Infographic
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In this infographic, you will see the following information for IL1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For IL-1-alpha Interleukin-1 alpha Porcine Recombinant Protein (PROTP18430)
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