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Product Info Summary
| SKU: | EK0596 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Mouse Adiponectin ELISA Kit PicoKine®
View all Adiponectin/Acrp30 ELISA kits
SKU/Catalog Number
EK0596
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Adiponectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Adiponectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0596)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E18-N244
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0596 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0596 is reactive to ADIPOQ in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK0596 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Adiponectin/Acrp30
Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0596-CAP | Anti-Mouse ADIPOQ Pre-coated 96-well strip microplate | 1 |
| EK0596-ST | Mouse ADIPOQ Standard | 2 vials, 20 ng/tube |
| EK0596-DA | Mouse ADIPOQ Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.000 | 0.149 | 0.298 | 0.541 | 0.897 | 1.274 | 1.546 | 2.083 |
Data Example Images
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Mouse Adiponectin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Adiponectin/Acrp30, Dilution ratio of 1:2000, concentration in serum and plasma is 4.8-6.1 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Adiponectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 567 | 2994 | 7572 | 512 | 3121 | 7518 |
| Standard deviation | 37.98 | 179.64 | 318.02 | 37.75 | 240.31 | 330.79 |
| CV (%) | 6.7% | 6% | 4.2% | 7.1% | 7.7% | 4.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Adiponectin/Acrp30 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 567 | 603 | 628 | 571 | 592 | 24.91 | 4.2% |
| Sample 2 | 2994 | 2867 | 2845 | 2816 | 2880 | 67.97 | 2.3% |
| Sample 3 | 7572 | 7510 | 8167 | 7413 | 7665 | 295.03 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For ADIPOQ (Source: Uniprot.Org, NCBI)
Gene Name
ADIPOQ
Full Name
Adiponectin
Weight
26.414kDa
Alternative Names
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein ADIPOQ ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28 adiponectin, C1Q and collagen domain containing adiponectin|30 kDa adipocyte complement-related protein|adipocyte complement-related 30 kDa protein|adipose most abundant gene transcript 1 protein|adipose specific collagen-like factor|gelatin-binding protein 28
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ADIPOQ, check out the ADIPOQ Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ADIPOQ: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Adiponectin ELISA Kit PicoKine® (EK0596)
Hello CJ!
EK0596 has been cited in 34 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Role of adiponectin in adipose tissue wound healing.
Glypican 4 may be involved in the adipose tissue redistribution in high‑fat feeding C57BL/6J mice with peroxisome proliferators‑activated receptor γ agonist rosiglitazone treatment
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE−/− Mice through the ROS/MAPK/NF-κB Pathway
Puerarin Protects Pancreatic β-Cells in Obese Diabetic Mice via Activation of GLP-1R Signaling
Oleic acid influences the adipogenesis of 3T3-L1 cells via DNA Methylation and may predispose to obesity and obesity-related disorders
Oral Administration of Resveratrol Alleviates Osteoarthritis Pathology in C57BL/6J Mice Model Induced by a High-Fat Diet
Effects of replacing wheat flour with detoxified ginkgo nut powder on lipid metabolism of obese C57BL/6J male mice
Anti-inflammatory and antioxidant effects of polyphenols extracted from Antirhea borbonica medicinal plant on adipocytes exposed to Porphyromonas gingivalis and Escherichia coli lipopolysaccharides
Porphyromonas gingivalis lipopolysaccharide induces pro-inflammatory adipokine secretion and oxidative stress by regulating Toll-like receptor-mediated signaling pathways and redox enzymes in adipocytes
Effectiveness of amiodarone in treatment of cutaneous leishmaniasis caused by Leishmania major
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Customer Reviews
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1 Reviews For Mouse Adiponectin ELISA Kit PicoKine®
0
Adiponectin Analysis in Mouse Serum and Culture Media--Sanjay Arora, North Dakota State University, Pharmaceutical Sciences, Graduate Student
Excellent
Source: Biocompare.com
| Application | ELISA |
|---|---|
| Sample | Serum, tissue homogenate |
| Detection | -- |
"I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels. The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped. Reliable estimation, good supplier, reasonable price. Will keep using it." (Customer’s Tip: "Dilute tissue homogenate 1:100, use serum directly.")
Sanjay Arora
Verified customer
Submitted 2019-05-21
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Mouse Adiponectin ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-11-04
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-11-04
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
W. Hernandez
Verified customer
Asked: 2020-06-25
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-06-25
Question
Can you confirm if EK0394,EK0568,EK0527,EK0411 and EK0596 are compatible with ELISA microplate washers?
Verified customer
Asked: 2019-05-30
Answer
Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.
Boster Scientific Support
Answered: 2019-05-30
Question
Can you confirm if the EK0596 is compatible with ELISA microplate washers?
Verified customer
Asked: 2019-05-30
Answer
Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.
Boster Scientific Support
Answered: 2019-05-30
Question
Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?
Verified customer
Asked: 2019-05-28
Answer
For the Mouse IL-1 Beta ELISA Kit PicoKine™ (EK0394), Mouse MCP-1 ELISA Kit PicoKine™ (EK0568), Mouse TNF Alpha ELISA Kit PicoKine™ (EK0527), Mouse IL-6 ELISA Kit PicoKine™ (EK0411) and Mouse Adiponectin ELISA Kit PicoKine™ (EK0596), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.
Boster Scientific Support
Answered: 2019-05-29
Question
Q: how can I thaw whole blood sample for Adiponectin ELISA after freezing?
G. Johnson
Verified customer
Asked: 2018-09-05
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Adiponectin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-09-05
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
J. Sunder
Verified customer
Asked: 2018-05-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-13
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Adiponectin using the EK0596 Mouse Adiponectin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-04-26
Question
Q: What is the optimal O.D. value for Adiponectin ELISA kit? I used your Adiponectin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Adiponectin even though the O.D. values are not very high?
B. Perez
Verified customer
Asked: 2018-01-09
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Adiponectin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Adiponectin that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-01-09
