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Product Info Summary
| SKU: | EK1296 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Angiopoietin-1 ELISA Kit PicoKine®
View all Angiopoietin-1 ELISA kits
SKU/Catalog Number
EK1296
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Angiopoietin-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Angpt1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Angiopoietin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1296)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: H16-F497
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1296 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1296 is reactive to ANGPT1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1296 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Angiopoietin-1
Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1296-CAP | Anti-Mouse ANGPT1 Pre-coated 96-well strip microplate | 1 |
| EK1296-ST | Mouse ANGPT1 Standard | 2 vials, 10 ng/tube |
| EK1296-DA | Mouse ANGPT1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.043 | 0.082 | 0.165 | 0.271 | 0.405 | 0.713 | 1.219 | 1.849 |
Data Example Images
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Mouse Angiopoietin-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Angiopoietin-1, Dilution ratio of 1:200, concentration in serum and plasma is around 300 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Angiopoietin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 351 | 1479 | 5183 | 351 | 1592 | 4701 |
| Standard deviation | 27.02 | 97.61 | 207.32 | 30.53 | 117.8 | 230.34 |
| CV (%) | 7.7% | 6.6% | 4% | 8.7% | 7.4% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 351 | 346 | 324 | 358 | 344 | 12.71 | 3.6% |
| Sample 2 | 1479 | 1733 | 1681 | 1766 | 1664 | 111.44 | 6.6% |
| Sample 3 | 5183 | 4874 | 5083 | 5078 | 5054 | 112.31 | 2.2% |
Protein Target Info & Infographic
Gene/Protein Information For ANGPT1 (Source: Uniprot.Org, NCBI)
Gene Name
ANGPT1
Full Name
Angiopoietin-1
Weight
57.513kDa
Alternative Names
AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003 ANGPT1 AGP1, AGPT, ANG1 angiopoietin 1 angiopoietin-1|ANG-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ANGPT1, check out the ANGPT1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ANGPT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Angiopoietin-1 ELISA Kit PicoKine® (EK1296)
Hello CJ!
EK1296 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Whole blood transfusion improves vascular integrity and increases survival in artemether-treated experimental cerebral malaria
Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4
Simvastatin ameliorates graft-vs-host disease by regulating angiopoietin-1 and angiopoietin-2 in a murine model
Blocking exosomal miRNA-153-3p derived from bone marrow mesenchymal stem cells ameliorates hypoxia-induced myocardial and microvascular damage by targeting the ANGPT1-mediated VEGF/PI3k/Akt/eNOS pathway
Platelets docking to VWF prevent leaks during leukocyte extravasation by stimulating Tie-2
Species: Mouse
Simvastatin ameliorates graft-vs-host disease by regulating angiopoietin-1 and angiopoietin-2 in a murine model
Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4
Schlosser K, Taha M, Deng Y, McIntyre LA, Mei SHJ, Stewart DJ. Thorax. 2018 Mar;73(3):248-261. doi: 10.1136/thoraxjnl-2017-210413. Epub 2017 Sep 25. High circulating angiopoietin-2 levels exacerbate pulmonary inflammation but not vascular leak or ...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
13 Customer Q&As for Mouse Angiopoietin-1 ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for Angiopoietin-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-04-04
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Angiopoietin-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-04-04
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
S. Babu
Verified customer
Asked: 2019-05-31
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-05-31
Question
Do you have any recommended buffer to make tissue lysate for EK1296?
Verified customer
Asked: 2019-04-08
Answer
Below is our sample preparation protocol for cell lysate for the Mouse Angiopoietin-1 ELISA Kit PicoKine™ (EK1296). Cell Lysate Sample Preparation: Collect and rinse cells in PBS. Homogenize and lyse cells thoroughly in lysis buffer (e.g. Mammal Cell Protein Extraction Reagent, Boster Catalog Number: AR0103). Centrifuge cell lysate at approximately 10,000 x g and 4°C for 5 min. Assay immediately or aliquot supernatant and hold at -80°C (Avoid freeze/thaw cycles). Guidelines on lysis buffer: Avoid more than 0.1% SDS or other strongly denaturing detergents (In general, non-ionic detergents such as Triton X-100 or NP-40 are best. Zwitterionic detergents such as CHAPS or mild ionic detergents such as sodium deoxycholate will work). Do not use more than 2% v/v total detergent. Avoid the use of sodium azide. Avoid more than 10 mM reducing agents (e.g. dithiothreitol, mercaptoethanols).
Boster Scientific Support
Answered: 2019-04-08
Question
Do you think there are some interfering substances such as detergent? We intend to use the lysis buffer below. Do you have any advise regarding the compatibility of the buffer to the EK1296 assay? Lysis Buffer Tris-Cl (pH 7.5) 50 mM NaCl 150 mM DTT 1 mM NP-40 0.5% Triton X-100 1% Sodium deoxycholate 1% SDS 0.1%
Verified customer
Asked: 2019-04-03
Answer
The lysis Buffer isn't compatible to the Mouse Angiopoietin-1 ELISA Kit PicoKine™ (EK1296) assay. Sodium deoxycholate 1% and SDS 0.1% will denature the protein.
Boster Scientific Support
Answered: 2019-04-03
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Anand
Verified customer
Asked: 2019-01-15
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-01-15
Question
Q: can you suggest the dilution ratio of serum samples for detection of Angiopoietin-1 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Angiopoietin-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-01-11
Question
Q: if the enzyme conjugated Angiopoietin-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Angiopoietin-1 antigen?
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-05-22
Question
Q: how to proceed with the analysis of ELISA data? I have performed Angiopoietin-1 level in plasma.
Y. Hernandez
Verified customer
Asked: 2018-05-15
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-15
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-01-26
Question
Does the antibody in this kit cross-react with ANGPT2?
Verified Customer
Verified customer
Asked: 2016-01-06
Answer
No, it does not.
Boster Scientific Support
Answered: 2016-01-06
Question
Q: can I use citrate plasma as samples in Mouse Angiopoietin-1 Picokine® ELISA Kit (Catalog # EK1296)?
Verified Customer
Verified customer
Asked: 2015-02-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Angiopoietin-1 causing degradation of its protein structure. Angiopoietin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Angiopoietin-1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-02-19
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2014-11-01
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-11-01
Question
Do you think mouse tissue lysate can be used with EK1296?
Verified Customer
Verified customer
Asked: 2013-09-16
Answer
We have not tested the Mouse Angiopoietin-1 ELISA Kit PicoKine™ (EK1296) with tissue lysates/homogenates before. For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit. • Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. • If no signal is produced, then you can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2013-09-16
