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Product Info Summary
| SKU: | EK0996 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse MMP-12 ELISA Kit PicoKine®
SKU/Catalog Number
EK0996
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MMP-12 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp12 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MMP-12 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0996)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A18-C462
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0996 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0996 is reactive to MMP12 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0996 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-12
Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0996-CAP | Anti-Mouse MMP12 Pre-coated 96-well strip microplate | 1 |
| EK0996-ST | Mouse MMP12 Standard | 2 vials, 10 ng/tube |
| EK0996-DA | Mouse MMP12 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.049 | 0.132 | 0.211 | 0.367 | 0.700 | 1.025 | 1.434 | 1.891 |
Data Example Images
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Mouse MMP-12 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-12, Dilution ratio of 1:1, concentration in serum and plasma is around 100 pg/ml..
Some articles we found to cite concentrations of MMP-12 in samples: 25652021 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MMP-12 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 127 | 628 | 1430 | 134 | 684 | 1370 |
| Standard deviation | 9.27 | 46.47 | 100.1 | 11.12 | 57.45 | 97.27 |
| CV (%) | 7.3% | 7.4% | 7% | 8.3% | 8.4% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-12 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 127 | 129 | 117 | 123 | 124 | 4.58 | 3.6% |
| Sample 2 | 628 | 722 | 630 | 605 | 646 | 44.82 | 6.9% |
| Sample 3 | 1430 | 1243 | 1362 | 1284 | 1329 | 71.95 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For MMP12 (Source: Uniprot.Org, NCBI)
Gene Name
MMP12
Full Name
Macrophage metalloelastase
Weight
54.002kDa
Superfamily
peptidase M10A family
Alternative Names
EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12 MMP12 HME, ME, MME, MMP-12 matrix metallopeptidase 12 macrophage metalloelastase|matrix metallopeptidase 12 (macrophage elastase)|matrix metalloproteinase 12 (macrophage elastase)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP12, check out the MMP12 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse MMP-12 ELISA Kit PicoKine® (EK0996)
Hello CJ!
EK0996 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
ADAM17 protects against elastase-induced emphysema by suppressing CD62L+ leukocyte infiltration in mice
Pulmonary C Fibers Modulate MMP-12 Production via PAR2 and Are Involved in the Long-Term Airway Inflammation and Airway Hyperresponsiveness Induced by Respiratory Syncytial Virus Infection
Pulmonary C Fibers Modulate MMP-12 Production via PAR2 and Are Involved in the Long-Term Airway Inflammation and Airway Hyperresponsiveness Induced by Respiratory Syncytial Virus Infection
Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection
Vaz M, Rajasekaran S, Potteti Hr, Reddy Sp. Am J Respir Cell Mol Biol. 2015 Jul;53(1):125-34. Doi: 10.1165/Rcmb.2014-0118Oc. Myeloid-Specific Fos-Related Antigen-1 Regulates Cigarette Smoke-Induced Lung Inflammation, Not Emphysema, In Mice.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
9 Customer Q&As for Mouse MMP-12 ELISA Kit PicoKine®
Question
Can EK0996 cross react with rat? If you have tested this, what samples types for tested?
Verified customer
Asked: 2020-11-09
Answer
Our lab hasn't validated cross reactivity with rats. The immunogen sequence of both capture and detection antibodies in the Mouse MMP-12 ELISA Kit PicoKine™ (EK0996) is A18-C462 Accession # P34960.
Boster Scientific Support
Answered: 2020-11-10
Question
Can EK0996 detect total MMP-12, just active MMP-12 or just Pro-MMP-12?
Verified customer
Asked: 2020-10-15
Answer
The immunogen sequence of both capture and detection antibodies in the Mouse MMP-12 ELISA Kit PicoKine™ (EK0996) is A18-C462 Accession # P34960.
Boster Scientific Support
Answered: 2020-10-16
Question
Q: how to analyze ELISA data? I have performed MMP-12 level in serum.
Verified Customer
Verified customer
Asked: 2020-09-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-09-09
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
W. Wang
Verified customer
Asked: 2020-06-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-06-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
L. Mehta
Verified customer
Asked: 2019-06-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-27
Question
Q: Can MMP-12 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-04-20
Question
Would this mouse ELISA work for human MMP-12? Do you sell the MMP-12 detection antibody that is specific for the human form only?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
No, the mouse kit would not work for human MMP-12 because there is no cross reactivity between the two species. Yes, although not in our product catalog at this time, you can buy the MMP-12 detection antibody alone from the kit.
Boster Scientific Support
Answered: 2018-02-05
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-10-05
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-10-05
Question
Q: is it okay to use citrate plasma as samples in Mouse MMP-12 Picokine® ELISA Kit (Catalog # EK0996)?
V. Varma
Verified customer
Asked: 2016-03-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of MMP-12 causing degradation of its protein structure. MMP-12 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MMP-12 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-03-09
