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Product Info Summary
| SKU: | FEK0582 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
| Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Mouse Resistin PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0582
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Resistin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Retn in cell culture supernatants, serum and plasma (heparin). . Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Resistin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0582)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S21-S114
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0582 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0582 is reactive to RETN in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
FEK0582 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Resistin
Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
Kit Components
| Catalog number | Description | Quantity |
|---|---|---|
| FEK0582-CAP | 96-well plate precoated with anti-Mouse Resistin antibody | 1 |
| EK0582-ST | lyophilized recombinant Mouse Resistin standard | 10 ng/tube |
| FEK0582-DA | Biotinylated anti-Mouse Resistin detection antibody (50x) | 100 ul |
| - | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
| AR1106-1 | Sample diluent | 30 ml |
| AR1106-2 | Antibody diluent | 12 ml |
| - | Avidin-Biotin-Peroxidase Diluent | 12 ml |
| AR1106-5 | Wash Buffer (25x) | 20 ml |
| AR1104 | Color Developing Reagent (TMB) | 10 ml |
| AR1105 | Stop Solution | 10 ml |
| PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.006 | 0.122 | 0.245 | 0.441 | 0.743 | 1.101 | 1.748 | 2.241 |
Data Example Images
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Mouse Resistin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 77 | 245 | 929 | 80 | 252 | 861 |
| Standard deviation | 6.2%2 | 13.23 | 57.59 | 7.28 | 17.64 | 69.74 |
| CV (%) | 7.7% | 5.4% | 6.2% | 9.1% | 7% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Resistin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 77 | 78 | 88 | 84 | 81 | 4.49 | 5.5% |
| Sample 2 | 245 | 263 | 267 | 252 | 256 | 8.72 | 3.4% |
| Sample 3 | 929 | 803 | 900 | 906 | 884 | 48.28 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For RETN (Source: Uniprot.Org, NCBI)
Uniprot ID
Q9HD89
Gene ID
56729
Gene Name
RETN
Full Name
Resistin
Weight
11.419kDa
Superfamily
resistin/FIZZ family
Alternative Names
Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1 RETN ADSF, FIZZ31, RSTN, XCP1, RETN resistin resistin|C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1|adipose tissue-specific secretory factor|c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein|cysteine-rich secreted protein A12-alpha-like 2|cysteine-rich secreted protein FIZZ3|found in inflammatory zone 3|resistin delta2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RETN, check out the RETN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RETN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Resistin PicoKine® Quick ELISA Kit (FEK0582)
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