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Product Info Summary
| SKU: | EK1996 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin) |
Product info
Product Name
Mouse SLPI ELISA Kit PicoKine®
SKU/Catalog Number
EK1996
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SLPI ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse SLPI in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SLPI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1996)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G26-M131
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1996 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1996 is reactive to SLPI in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin)
Application Guarantee
EK1996 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SLPI
Antileukoproteinase, also known as secretory leukocyte protease inhibitor (SLPI), is an enzyme that in humans is encoded by the SLPI gene. It is mapped to 2; 2 H3. SLPI is a highly cationic single-chain protein with eight intramolecular disulfide bonds. It is found in large quantities in bronchial, cervical, and nasal mucosa, saliva, and seminal fluids. SLPI inhibits human leukocyte elastase, human cathepsin G, human trypsin, neutrophil elastase, and mast cell chymase. X-ray crystallography has shown that SLPI has two homologous domains of 53 and 54 amino acids, one of which exhibits anti-protease activity (C-terminal domain). The other domain (N-terminal domain) is not known to have any function.This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1996-CAP | Anti-Mouse SLPI Pre-coated 96-well strip microplate | 1 |
| EK1996-ST | Mouse SLPI Standard | 2 vials, 2 ng/tube |
| EK1996-DA | Mouse SLPI Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.018 | 0.107 | 0.197 | 0.414 | 0.769 | 1.330 | 1.769 | 2.063 |
Data Example Images
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Mouse SLPI PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SLPI, Dilution ratio of 1:4000, concentration in serum and plasma is 1.6-6.4 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SLPI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 59 | 214 | 938 | 62 | 237 | 958 |
| Standard deviation | 2.48 | 11.34 | 53.47 | 3.84 | 13.75 | 60.35 |
| CV (%) | 4.2% | 5.3% | 5.7% | 6.2% | 5.8% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SLPI in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 59 | 56 | 65 | 66 | 61 | 4.15 | 6.8% |
| Sample 2 | 214 | 215 | 194 | 223 | 211 | 10.68 | 5.0% |
| Sample 3 | 938 | 982 | 1040 | 958 | 979 | 38.24 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For SLPI (Source: Uniprot.Org, NCBI)
Gene Name
SLPI
Full Name
Antileukoproteinase
Weight
14.326kDa
Alternative Names
ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; SLPI; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI SLPI ALK1, ALP, BLPI, HUSI, HUSI-I, MPI, WAP4, WFDC4 secretory leukocyte peptidase inhibitor antileukoproteinase|HUSI-1|WAP four-disulfide core domain protein 4|mucus proteinase inhibitor|protease inhibitor WAP4|secretory leukocyte protease inhibitor (antileukoproteinase)|seminal proteinase inhibitor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SLPI, check out the SLPI Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SLPI: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SLPI ELISA Kit PicoKine® (EK1996)
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No publications found for EK1996
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Mouse SLPI ELISA Kit PicoKine®
Question
Q: how to analyze ELISA data? I measured SLPI level in plasma.
Verified Customer
Verified customer
Asked: 2020-10-13
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-10-13
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
C. Mehta
Verified customer
Asked: 2020-03-13
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-13
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-12-22
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-22
Question
Q: if the enzyme conjugated SLPI antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SLPI antigen?
M. Dhawan
Verified customer
Asked: 2019-10-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-10-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-08-08
Question
Q: can I use citrate plasma as samples in Mouse SLPI Picokine® ELISA Kit (Catalog # EK1996)?
Verified Customer
Verified customer
Asked: 2018-09-08
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of SLPI causing degradation of its protein structure. SLPI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the SLPI ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-09-08
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of SLPI in Mouse plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-03-14
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SLPI ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-03-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Johnson
Verified customer
Asked: 2014-11-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-11-16
