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Product Info Summary
| SKU: | EK1515 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse TNFRSF25/DR3 ELISA Kit PicoKine®
View all DR3/TNFRSF25 ELISA kits
SKU/Catalog Number
EK1515
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TNFRSF25/DR3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNFRSF25/DR3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1515)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q31-F199
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1515 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1515 is reactive to TNFRSF25 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1515 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DR3/TNFRSF25
Death receptor 3 (DR3), also known as tumor necrosis factor receptor superfamily member 25 (TNFRSF25), is a cell surface receptor of the tumor necrosis factor receptor superfamily which mediates apoptotic signalling and differentiation. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1515-CAP | Anti-Mouse TNFRSF25 Pre-coated 96-well strip microplate | 1 |
| EK1515-ST | Mouse TNFRSF25 Standard | 2 vials, 10 ng/tube |
| EK1515-DA | Mouse TNFRSF25 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10000 |
| O.D. | 0.041 | 0.092 | 0.122 | 0.195 | 0.401 | 0.847 | 1.741 | 2.402 |
Data Example Images
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Mouse TNFRSF25/DR3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect DR3/TNFRSF25, Dilution ratio of 1:1, concentration in serum and plasma is around 1000 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNFRSF25/DR3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 206 | 1289 | 4332 | 201 | 1293 | 3972 |
| Standard deviation | 8.44 | 68.31 | 316.23 | 11.65 | 68.52 | 317.76 |
| CV (%) | 4.1% | 5.3% | 7.3% | 5.8% | 5.3% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DR3/TNFRSF25 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 206 | 209 | 222 | 189 | 206 | 11.75 | 5.7% |
| Sample 2 | 1289 | 1163 | 1209 | 1152 | 1203 | 53.92 | 4.4% |
| Sample 3 | 4332 | 4412 | 4054 | 4654 | 4363 | 214.19 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF25 (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF25
Full Name
Tumor necrosis factor receptor superfamily member 25
Weight
45.385kDa
Alternative Names
APO3; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; DR3; DR3member 12 (translocatingchain-association membrane protein); LARD; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; TNFRSF25; TRAMP; tumor necrosis factor receptor superfamily, member 25; WSL; WSL1; WSL-1 TNFRSF25 APO-3, DDR3, DR3, GEF720, LARD, PLEKHG5, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR TNF receptor superfamily member 25 tumor necrosis factor receptor superfamily member 25|Guanine nucleotide exchange factor 720|PH domain-containing family G member 5|Pleckstrin homology domain-containing family G member 5|apoptosis inducing receptor|apoptosis-inducing receptor AIR|apoptosis-mediating receptor DR3|apoptosis-mediating receptor TRAMP|death domain receptor 3 soluble form|death receptor beta|lymphocyte-associated receptor of death|protein WSL-1|tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF25, check out the TNFRSF25 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF25: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TNFRSF25/DR3 ELISA Kit PicoKine® (EK1515)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Mouse TNFRSF25/DR3 ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I have performed DR3 level in plasma.
M. Li
Verified customer
Asked: 2020-06-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-06-18
Question
Q: if the enzyme conjugated DR3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DR3 antigen?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-01-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
L. Smith
Verified customer
Asked: 2019-09-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-09-30
Question
Q: how can I thaw whole blood sample for DR3 ELISA after freezing?
R. Huang
Verified customer
Asked: 2019-05-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test DR3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-05-11
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
C. Jones
Verified customer
Asked: 2018-07-30
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-07-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Edwards
Verified customer
Asked: 2018-04-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-05
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring DR3 using the EK1515 Mouse DR3 Picokine® ELISA Kit?
N. Gonzalez
Verified customer
Asked: 2016-08-22
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2016-08-22
