Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine™ Quick ELISA Kit

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Product Info Summary

SKU: FEK0960
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Assay Time: 90 Minutes

Product Name

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine™ Quick ELISA Kit

See all Angiopoietin-like Protein 4/ANGPTL4 products

SKU/Catalog Number

FEK0960

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human ANGPTL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human ANGPTL4/Angiopoietin Like 4/ARP4 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0960)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L165-S406

Sensitivity

<20 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0960 is reactive to ANGPTL4 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

FEK0960 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK0960-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0960-STHuman Angiopoietin-like Protein 4/ANGPTL4 Standard 2 40 ng/tube
FEK0960-CHuman ANGPTL4 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D.0.0130.1590.2520.3950.6200.9911.5022.064

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12104752210311116471221659
Standard deviation81.07261.36967.4381.47325.13974.66
CV (%)6.7%5.5%4.6%7.3%6.9%4.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Angiopoietin-like Protein 4/ANGPTL4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11210112813021147119774.26.2%
Sample 247524862514545104817269.775.6%
Sample 32103121450204861884320453859.014.2%
*number of samples for each test n=16.

Gene/Protein Information For ANGPTL4 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9BY76

Gene ID

51129

Gene Name

ANGPTL4

Full Name

Angiopoietin-related protein 4

Weight

45.214kDa

Alternative Names

Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein ANGPTL4 ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158 angiopoietin like 4 angiopoietin-related protein 4|PPARG angiopoietin related protein|fasting-induced adipose factor|hepatic angiopoietin-related protein|hepatic fibrinogen/angiopoietin-related protein|peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ANGPTL4, check out the ANGPTL4 Infographic

ANGPTL4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ANGPTL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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