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Product Info Summary
| SKU: | EK0540 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Rat VEGF ELISA Kit PicoKine®
SKU/Catalog Number
EK0540
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat VEGF ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat VEGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0540)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A27-R190
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0540 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0540 is reactive to VEGFA in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0540 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFA
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0540-CAP | Anti-Rat VEGFA Pre-coated 96-well strip microplate | 1 |
| EK0540-ST | Rat VEGFA Standard | 2 vials, 10 ng/tube |
| EK0540-DA | Rat VEGFA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.045 | 0.065 | 0.128 | 0.212 | 0.343 | 0.660 | 1.159 | 1.889 |
Data Example Images
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Rat VEGF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat VEGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 29 | 243 | 389 | 31 | 234 | 368 |
| Standard deviation | 1.91 | 18.95 | 16.72 | 2.07 | 16.75 | 17.66 |
| CV (%) | 6.6% | 7.8% | 4.3% | 6.7% | 7.8% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of VEGFA in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 28 | 31 | 28 | 29 | 1.22 | 4.2% |
| Sample 2 | 243 | 226 | 269 | 260 | 249 | 16.46 | 6.6% |
| Sample 3 | 389 | 381 | 403 | 431 | 401 | 19.02 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For VEGFA (Source: Uniprot.Org, NCBI)
Gene Name
VEGFA
Full Name
Vascular endothelial growth factor A
Weight
27.042kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor VEGFA MVCD1, VEGF, VPF vascular endothelial growth factor A vascular endothelial growth factor A|vascular endothelial growth factor A121|vascular endothelial growth factor A165|vascular permeability factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on VEGFA, check out the VEGFA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for VEGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat VEGF ELISA Kit PicoKine® (EK0540)
Hello CJ!
EK0540 has been cited in 96 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of Endoglin and Vascular Endothelial Growth Factor as Prognostic Markers in Experimental Colorectal Cancer
Biocompatibility of porcine small intestinal submucosa and rat endothelial progenitor cells in vitro
A novel tissue engineered nerve graft constructed with autologous vein and nerve microtissue repairs a long-segment sciatic nerve defect
Long-Term Running Exercise Delays Age-Related Changes in White Matter in Rats
Inhibitory Effect of Sub-conjunctival Tocilizumab on Alkali Burn Induced Corneal Neovascularization in Rats
NLRP3 Blockade Suppresses Pro-Inflammatory and Pro-Angiogenic Cytokine Secretion in Diabetic Retinopathy
Regular aerobic exercise increased VEGF levels in both soleus and gastrocnemius muscles correlated with hippocampal learning and VEGF levels.
The antiiflammatory effect of Ginkgo biloba in lipopolysaccharide-induced rat sepsis model
Losartan and Pioglitazone Ameliorate Nephropathy in Experimental Metabolic Syndrome Rats
PTEN inhibitor VO-OHpic attenuates GC-associated endothelial progenitor cell dysfunction and osteonecrosis of the femoral head via activating Nrf2 signaling and inhibiting mitochondrial apoptosis pathway
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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18 Customer Q&As for Rat VEGF ELISA Kit PicoKine®
Question
Does 10% FBS or phenol red in complete cell culture media affect EK0540?
Verified customer
Asked: 2020-12-17
Answer
10% FBS or phenol red in complete cell culture media doesn't affect the assay using the Rat VEGF ELISA Kit PicoKine™ (EK0540).
Boster Scientific Support
Answered: 2020-12-18
Question
Does EK0540 by any chance recognize the mouse version? If not, do you have a kit that would recognize both rat and mouse (or even human)?
Verified customer
Asked: 2020-12-17
Answer
No, the Rat VEGF ELISA Kit PicoKine™ (EK0540) can't recognize the mouse version. We haven't got any kit that could recognize both rat and mouse.
Boster Scientific Support
Answered: 2020-12-18
Question
What is the recipe for the color developing reagent (TMB) for EK0540? I spilled some and would like to make up for what is lost, so the entire kit does not get wasted.
Verified customer
Asked: 2020-12-17
Answer
Unfortunately, we cannot disclose the recipe of the color developing reagent (TMB) for Rat VEGF ELISA Kit PicoKine™ (EK0540), but we can provide a free bottle to the customer.
Boster Scientific Support
Answered: 2020-12-18
Question
In the EK0540 protocol there is no wash step between step number 5 (removal of sample following 120 min incubation) and step number 6 (addition of biotinylated Anti-Rat VEGF), was wondering if the wash steps were not necessary or omitted by mistake?
Verified customer
Asked: 2020-06-30
Answer
For the Rat VEGF ELISA Kit PicoKine™ (EK0540), there is no wash step between step number 5 (removal of sample following 120 min incubation) and step number 6 (addition of biotinylated Anti-Rat VEGF). Our lab technicians found there wasn't any significant difference in result values between washing and without washing the plate after incubating samples/standard and before addition of the detection antibody. It is to make the protocol easy and fast.
Boster Scientific Support
Answered: 2020-07-01
Question
What is the recipe for the color developing reagent (TMB) for EK0540? I spilled some and would like to make up for what is lost, so the entire kit does not get wasted.
Verified customer
Asked: 2020-01-17
Answer
Unfortunately, we cannot disclose the recipe of the color developing reagent (TMB) for Rat VEGF ELISA Kit PicoKine™ (EK0540), but we can provide a free bottle to the customer.
Boster Scientific Support
Answered: 2020-01-18
Question
Can I use the RIPA lysis buffer with this kit?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Can I use the RIPA lysis buffer with this kit? Yes. You can use this buffer to prepare for your tissue homogenates.
Boster Scientific Support
Answered: 2020-01-08
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis VEGFA using the EK0540 Rat VEGFA Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-12-16
Question
Does the stop solution of EK0540 contain acid? If so, what is the percentage and name?
Verified customer
Asked: 2019-12-02
Answer
The stop solution for the Rat VEGF ELISA Kit PicoKine™ (EK0540) is 2% sulfuric acid.
Boster Scientific Support
Answered: 2019-12-02
Question
Were there any viral vector used in Rat VEGF ELISA Kit PicoKine?
Verified customer
Asked: 2019-11-26
Answer
There wasn't any viral vectors used in Rat VEGF ELISA Kit PicoKine, only CHO expression system was used.
Boster Scientific Support
Answered: 2019-11-26
Question
Q: Can VEGFA ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-06-24
Question
What are the differences and advantages between your EK0540 kit from RandD RRV00 kit?
Verified customer
Asked: 2019-03-20
Answer
Our Rat VEGF ELISA Kit PicoKine™ (EK0540) has a different sensitivity and assay range compared to the RandD RRV00 kit, so you should make sure you get the kit that would fit your experimental needs.
Boster Scientific Support
Answered: 2019-03-20
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Y. Sunder
Verified customer
Asked: 2018-04-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-12
Question
Dear Support: we purchased Rat VEGF ELISA Kit PicoKine to assay VEGF concentration in DRG tissues and it works well, however, when we measured the tissue lysate protein concentration by using BCA protein assay kit, it did not work. Please give us advice on how to assay the tissue lysate protein concentration. Thanks.
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
The BCA total protein assay is fine with our lysis buffer. Actually we are using it all the time in our lab to determine the total protein in lysate samples.
Boster Scientific Support
Answered: 2017-06-02
Question
Q: if the enzyme conjugated VEGFA antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the VEGFA antigen?
Verified Customer
Verified customer
Asked: 2016-12-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-12-30
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2016-10-24
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-10-24
Question
Does EK0540 detect other isoforms of Rat VEGF?
Verified Customer
Verified customer
Asked: 2016-09-27
Answer
There are four isoforms for rat VEGF. https://www.uniprot.org/uniprot/P16612#sequences EK0540 is able to detect all of them.
Boster Scientific Support
Answered: 2016-09-27
Question
Q: What is the optimal O.D. value for VEGFA ELISA kit? I used your VEGFA ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain VEGFA even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-08-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this VEGFA ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of VEGFA that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2016-08-30
Question
Is this product suitable for rat alveolar bone samples? Keywords: protocol, sample type, applications
Verified Customer
Verified customer
Asked: 2014-12-09
Answer
EK0540 can be used to detect the rat alveolar bone samples if the concentraion of VEGF in the sample is in the detection range (15.6pg/ml-1000pg/ml) of the ELISA kit. Here is the sample preparation method of general bone tissue for your reference: Extract demineralized bone samples in 4 M Guanidine-HCl and protease inhibitors. Dissolve the final sample in 2 M Guanidine-HCl. The ELISA kit protocol can be found on the datasheet of EK0540, which is downloadable as a PDF on the product's webpage.
Boster Scientific Support
Answered: 2014-12-09
