TNFSF12 TNF Ligand Superfamily Member 12 Human Recombinant Protein

Product Name

TNFSF12 TNF Ligand Superfamily Member 12 Human Recombinant Protein

View all TWEAK/TNFSF12 recombinant proteins

SKU/Catalog Number

PROTO43508

Size

5ug, 25ug, 1mg

Description

TNFSF12 Human Recombinant (94-249 a.a.) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a total molecular mass of 18kDa. The TNFSF12 is fused with an 8 amino acids his tag at N-terminal (M-HHHHHH-R, total 164 a.a.) and purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized TNFSF12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TWEAK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

TNFSF12 TNF Ligand Superfamily Member 12 Human Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTO43508)

Form

Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS.

Purity

Greater than 95% as determined by SDS-PAGE.

Predicted MW

27.216kDa

Reconstitution

It is recommended to reconstitute the lyophilized TNFSF12 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED₅₀, as measured in a proliferation assay using HUVECs, is less than 8ng/ml.

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Reconstitution

It is recommended to reconstitute the lyophilized TNFSF12 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For TNFSF12 (Source: Uniprot.org, NCBI)

Alternative Names

APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK TNFSF12 APO3L, DR3LG, TNLG4A, TWEAK TNF superfamily member 12 tumor necrosis factor ligand superfamily member 12|APO3 ligand|APO3/DR3 ligand|TNF-related WEAK inducer of apoptosis|tumor necrosis factor (ligand) superfamily, member 12|tumor necrosis factor ligand 4A|tumor necrosis factor superfamily member 12

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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