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Product Info Summary
| SKU: | A32410 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ADAP FYB1 Antibody
View all FYB/ADAP/SLAP130 Antibodies
SKU/Catalog Number
A32410
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ADAP FYB1 Antibody (Catalog # A32410). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ADAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ADAP FYB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32410)
Host
Rabbit
Contents
ADAP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ADAP antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human ADAP. The immunogen is located within amino acids 640 - 690 of ADAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of HDGFRP2 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other HDGFRP family members
Reactive Species
A32410 is reactive to FYB1 in Human, Mouse
Applications
A32410 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
85.387kDa
Background of FYB/ADAP/SLAP130
The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-κB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For FYB1 (Source: Uniprot.org, NCBI)
Gene Name
FYB1
Full Name
FYN-binding protein 1
Weight
85.387kDa
Alternative Names
ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130SLAP-130FYN-binding protein (FYB-120/130); FYN binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP130; SLP-76-associated phosphoprotein FYB1 ADAP, FYB, PRO0823, SLAP-130, SLAP130, THC3 FYN binding protein 1 FYN-binding protein 1|FYB-120/130|FYN-T-binding protein|SLP-76-associated phosphoprotein|adhesion and degranulation-promoting adaptor protein|p120/p130
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FYB1, check out the FYB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FYB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAP FYB1 Antibody (A32410)
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No publications found for A32410
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