Anti-AKT3 Antibody Picoband™

Product Name

Anti-AKT3 Antibody Picoband™

View all AKT3 Antibodies

SKU/Catalog Number

A00520-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AKT3 Antibody Picoband™ catalog # A00520-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-AKT3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00520-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human AKT3 recombinant protein (Position: A104-L154).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00520-2 is reactive to AKT3 in Human, Mouse, Rat

Applications

A00520-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

55.775kDa

Background of AKT3

RAC-gamma serine/threonine-protein kinase, also known as protein kinase Akt-3, is an enzyme that in humans is encoded by the AKT3 gene. This gene is mapped to 1q43-q44. The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor(PDGF), insulin, and insulin-like growth factor 1(IGF1). AKT3 plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. This gene is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of AKT3 using anti-AKT3 antibody (A00520-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3 mouse brain tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates,
Lane 6: human SH-SY5Y whole cell lysates,
Lane 7: human U-87MG whole cell lysates,
Lane 8: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (Catalog # A00520-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 60 kDa. The expected band size for AKT3 is at 56 kDa.

Gene/Protein Information For AKT3 (Source: Uniprot.org, NCBI)

Alternative Names

Akt3; Akt-3; EC 2.7.11; EC 2.7.11.1; PKB gamma; PKBGDKFZp434N0250; PRKBG; RAC-gamma; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) AKT3 MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2 AKT serine/threonine kinase 3 RAC-gamma serine/threonine-protein kinase|PKB gamma|RAC-gamma serine/threonine protein kinase|v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

For more info on AKT3, check out the AKT3 Infographic

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In this infographic, you will see the following information for AKT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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