Anti-APC1 phospho S355 mat-2 Antibody

Product Name

Anti-APC1 phospho S355 mat-2 Antibody

View all Apc1 Antibodies

SKU/Catalog Number

P03471-2

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-APC1 phospho S355 mat-2 Antibody (Catalog # P03471-2). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-APC1 phospho S355 mat-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03471-2)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P03471-2 is reactive to ANAPC1 in Human

Applications

P03471-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

216.5kDa

Background of Apc1

APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:35,000
IP: 1:100
WB: 1:200 - 1:2,000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ANAPC1, TSG24 using anti-ANAPC1, TSG24 antibody (P03471-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANAPC1, TSG24 antigen affinity purified polyclonal antibody (Catalog # P03471-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ANAPC1, TSG24.

Gene/Protein Information For ANAPC1 (Source: Uniprot.org, NCBI)

Alternative Names

anaphase promoting complex subunit 1; anaphase-promoting complex 1 (meiotic checkpoint regulator); anaphase-promoting complex subunit 1; APC1Testis-specific gene 24 protein; Cyclosome subunit 1; Mitotic checkpoint regulator; TSG24MCPR ANAPC1 APC1, MCPR, TSG24 anaphase promoting complex subunit 1 anaphase-promoting complex subunit 1|anaphase-promoting complex 1 (meiotic checkpoint regulator)|cyclosome subunit 1|mitotic checkpoint regulator|testis-specific gene 24 protein

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