Anti-Apolipoprotein L1 ApoL1 Antibody

Product Name

Anti-Apolipoprotein L1 ApoL1 Antibody

View all Apolipoprotein L1 Antibodies

SKU/Catalog Number

A01841

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Apolipoprotein L1 ApoL1 Antibody catalog # A01841. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apolipoprotein L1 ApoL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01841)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human ApoL1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01841 is reactive to APOL1 in Human

Applications

A01841 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

43.974kDa

Background of Apolipoprotein L1

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.

Mittaz L., Genomics 41:210-217(1997).
Lai F., Mol. Cell. Biol. 17:2413-2424(1997).
Villard L., Somat. Cell Mol. Genet. 23:135-145(1997).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of APOL1 using Anti-Apolipoprotein L1 ApoL1 Antibody (A01841).

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C

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Figure 1. Western blotting validation for Anti-Apolipoprotein L1 ApoL1 Antibody A01841

Western Blot (WB) analysis of specific cells using ApoL1 polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For APOL1 (Source: Uniprot.org, NCBI)

Alternative Names

APOL; APO-L; APOL-I; Apolipoprotein L; apolipoprotein L, 1; apolipoprotein L1; Apolipoprotein L-I; FSGS4 APOL1 APO-L, APOL, APOL-I, FSGS4 apolipoprotein L1 apolipoprotein L1|apolipoprotein L 1|apolipoprotein L1-B3

For more info on APOL1, check out the APOL1 Infographic

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In this infographic, you will see the following information for APOL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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