Click image to see more details
Product Info Summary
| SKU: | PA1537 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ATF4 Antibody
SKU/Catalog Number
PA1537
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATF4 Antibody catalog # PA1537. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1537)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ATF4, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1537 is reactive to ATF4 in Human, Mouse, Rat
Applications
PA1537 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.59kDa
Background of ATF4
ATF4, Activating Transcription Factor 4, is also know as CREB2. ATF4 belongs to the large ATF/CREB family of transcription factors which bind DNA via their basic region and dimerize via their leucine zipper domain to form a variety of homo- and heterodimers to regulate gene transcription. It is identified that members of this family share significant sequence similarity within a leucine zipper DNA-binding motif and an adjacent basic region. The ATF4 gene is mapped to chromosome 22. Unlike CREB, which activates transcription from CRE-containing promoters, CREB2 functions as a specific repressor of CRE-dependent transcription. The transcriptional repressor activity resides within the C-terminal leucine zipper and basic domain region of the CREB2 protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-ATF4 antibody, PA1537, Western blotting
All lanes: Anti ATF4 (PA1537) at 0.5ug/ml
Lane 1: A431 Whole Cell Lysate at 40ug
Lane 2: RAJI Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
Protein Target Info & Infographic
Gene/Protein Information For ATF4 (Source: Uniprot.org, NCBI)
Gene Name
ATF4
Full Name
Cyclic AMP-dependent transcription factor ATF-4
Weight
38.59kDa
Superfamily
bZIP family
Alternative Names
activating transcription factor 4 (tax-responsive enhancer element B67); Activating transcription factor 4; ATF4; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2DNA-binding protein TAXREB67; cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; TAXREB67; TAXREB67CREB2cAMP response element-binding protein 2; Tax-responsive enhancer element-binding protein 67; TXREB ATF4 CREB-2, CREB2, TAXREB67, TXREB activating transcription factor 4 cyclic AMP-dependent transcription factor ATF-4|DNA-binding protein TAXREB67|cAMP response element-binding protein 2|cAMP-dependent transcription factor ATF-4|cAMP-responsive element-binding protein 2|cyclic AMP-responsive element-binding protein 2|tax-responsive enhancer element B67|tax-responsive enhancer element-binding protein 67
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF4, check out the ATF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF4 Antibody (PA1537)
Hello CJ!
PA1537 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-ATF4 Antibody
Question
We have seen staining in mouse leukemic t-cell. Are there any suggestions? Is anti-ATF4 antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Based on literature leukemic t-cell does express ATF4. Based on Uniprot.org, ATF4 is expressed in lower esophagus, fibroblast, leukemic t-cell, colon, lung, placenta urinary bladder, cervix carcinoma, among other tissues. Regarding which tissues have ATF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 20068231
Colon, Lung, Placenta, and Urinary bladder, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 1847461
Leukemic T-cell, Pubmed ID: 1534408
Boster Scientific Support
Answered: 2020-04-02
Question
Our lab were happy with the WB result of your anti-ATF4 antibody. However we have been able to see positive staining in placenta urinary bladder cytoplasm. using this antibody. Is that expected? Could you tell me where is ATF4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Based on literature, placenta urinary bladder does express ATF4. Generally ATF4 expresses in cytoplasm. Regarding which tissues have ATF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 20068231
Colon, Lung, Placenta, and Urinary bladder, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 1847461
Leukemic T-cell, Pubmed ID: 1534408
Boster Scientific Support
Answered: 2019-07-22
Question
My lab would like using your anti-ATF4 antibody for cellular calcium ion homeostasis studies. Has this antibody been tested with western blotting on a431 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-03-20
Answer
Thanks for your inquiry. This PA1537 anti-ATF4 antibody is validated on a431 whole cell lysate, raji whole cell lysate, cem whole cell lysate, hut whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-03-20
Question
We are currently using anti-ATF4 antibody PA1537 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
M. Li
Verified customer
Asked: 2017-11-09
Answer
The anti-ATF4 antibody (PA1537) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-09
