Anti-Axin-2 AXIN2 Antibody

Product Name

Anti-Axin-2 AXIN2 Antibody

View all Axin-2 Antibodies

SKU/Catalog Number

A01772

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Axin-2 AXIN2 Antibody (Catalog # A01772). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

AXIN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Axin-2 AXIN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01772)

Host

Rabbit

Contents

AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2. The immunogen is located within amino acids 780 - 830 of AXIN2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.

Reactive Species

A01772 is reactive to AXIN2 in Human, Mouse, Rat

Applications

A01772 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

93.558kDa

Background of Axin-2

Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 μg/mL.

Click image to see more details

Immunohistochemistry of AXIN2 in rat lung tissue with AXIN2 antibody at 5 μg/mL.

Click image to see more details

Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2 antibody at 20 μg/mL.

Click image to see more details

Immunohistochemistry of AXIN2 in mouse lung tissue with AXIN2 antibody at 5 μg/mL.

Click image to see more details

Immunofluorescence of AXIN2 in mouse lung tissue with AXIN2 antibody at 20 μg/mL.

Red: AXIN2 Antibody (A01772)
Blue: DAPI staining

Click image to see more details

Immunofluorescence of AXIN2 in HepG2 cells with AXIN2 Antibody at 20 μg/ml.

Gene/Protein Information For AXIN2 (Source: Uniprot.org, NCBI)

Alternative Names

AXIL; axin 2; Axin2; Axin-2; Axin-like protein; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582 AXIN2 AXIL, ODCRCS axin 2 axin-2|axin-like protein|axis inhibition protein 2|conductin

For more info on AXIN2, check out the AXIN2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AXIN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the AXIN2 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis