Anti-CAD Dffb Antibody

DFF40/CAD antibody

Boster Bio Anti-CAD Dffb Antibody (Catalog# A05374). Tested in IHC-P, IF (paraffin), WB applications. This antibody reacts with Human, Rat, Mouse.

Product Info Summary

SKU: A05374
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC-P, WB, IF (paraffin)

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Product Name

Anti-CAD Dffb Antibody

View all DFF40/CAD Antibodies

SKU/Catalog Number

A05374

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CAD Dffb Antibody (Catalog# A05374). Tested in IHC-P, IF (paraffin), WB applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CAD Dffb Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05374)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human CAD. Immunogen sequence location: 289 - 338

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05374 is reactive to DFFB in Human, Mouse, Rat

Applications

A05374 is guaranteed for IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

39.11kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC-P, 1:50 - 1:200
Western Blot, 1:500 - 1:2000

Validation Images & Assay Conditions

Gene/Protein Information For DFFB (Source: Uniprot.org, NCBI)

Gene Name

DFFB

Full Name

DNA fragmentation factor subunit beta

Weight

39.11kDa

Alternative Names

CAD; CADCaspase-activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; CPAN; CPANDNA fragmentation factor 40 kDa subunit; DFF2; DFF40; DFF-40DFF40DFF2DNA fragmentation factor subunit beta; DFFB; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); EC 3.- DFFB CAD, CPAN, DFF-40, DFF2, DFF40 DNA fragmentation factor subunit beta DNA fragmentation factor subunit beta|DNA fragmentation factor 40 kDa subunit|DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)|caspase-activated DNase|caspase-activated deoxyribonuclease|caspase-activated nuclease

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DFFB, check out the DFFB Infographic

DFFB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DFFB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-CAD Dffb Antibody

Question

We have observed staining in human cerebellar hemisphere. What should we do? Is anti-CAD antibody supposed to stain cerebellar hemisphere positively?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

From what I have seen in literature cerebellar hemisphere does express DFFB. From what I have seen in Uniprot.org, DFFB is expressed in cerebellar hemisphere, pancreas, fetal brain, lung, among other tissues. Regarding which tissues have DFFB expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Pancreas, Pubmed ID: 9560346

Boster Scientific Support

Answered: 2020-04-30

Question

We are currently using anti-CAD antibody A05374 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

The anti-CAD antibody (A05374) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-03

Question

We were content with the WB result of your anti-CAD antibody. However we have observed positive staining in pancreas cytoplasm. nucleus. using this antibody. Is that expected? Could you tell me where is DFFB supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

From literature, pancreas does express DFFB. Generally DFFB expresses in cytoplasm. nucleus. Regarding which tissues have DFFB expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334
Pancreas, Pubmed ID: 9560346

Boster Scientific Support

Answered: 2019-05-21

Question

Our lab want to know about using your anti-CAD antibody for protein homooligomerization studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.

K. Lewis

Verified customer

Asked: 2018-12-05

Answer

I appreciate your inquiry. This A05374 anti-CAD antibody is tested on jurkat cells. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-12-05

Order DetailsPrice
A05374

100 μL

$335

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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