Anti-Calbindin Monoclonal Antibody


SKU M03047-1
Size 100ul
Reactivity Bovine, Human, Mouse, Pig, Rat
Clonality Monoclonal
Host Mouse
Applications IF, IHC, WB

Overview

Product Name Anti-Calbindin Monoclonal Antibody
SKU/Catalog Number M03047-1
Storage & Handling Stable at 4℃ for one year, for longer term store at -20℃
Size 100ul
Description Anti-Calbindin Monoclonal Antibody tested in WB, ICC, IF, IHC, reactive to Human, rat, mouse, cow, pig
Cite This Product Anti-Calbindin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03047-1)
Host Mouse
Contents/Buffer Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Immunogen Full-length recombinant human calbindin protein expressed in and purified from E.Coli.
Reactivity Bovine, Human, Mouse, Pig, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB: 1:1,000-1:5,000. ICC/IF or IHC: 1:1,000.

Images And Assay Conditions

Western blot analysis of different neuronal tissue lysates using mouse mAb to calbindin, M03047-1, dilution 1:2,000: [1] protein standard,

Immunofluorescent analysis of rat brain cerebellum section stained with mouse mAb to calbindin, M03047-1, dilution 1:1,000, in green, and costained with rabbit pAb to calretinin, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45?M, and free-floating sections were stained with the above antibodies. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P05937
Gene Name CALB1
Protein Name Calbindin
Alternative Names Calbindin;Calbindin D28;D-28K;Vitamin D-dependent calcium-binding protein, avian-type;CALB1;CAB27;
Molecular Weight 30025 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Research Areas Bovine, Human, Mouse, Pig, Rat

*You can search these to find other products in these research areas.

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Anti-Calbindin Monoclonal Antibody tested in WB, ICC, IF, IHC, reactive to Human, rat, mouse, cow, pig
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In stock
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M03047-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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