Anti-CAP1 Antibody Picoband™

Product Name

Anti-CAP1 Antibody Picoband™

View all CAP1 Antibodies

SKU/Catalog Number

PB9923

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CAP1 Antibody Picoband™ catalog # PB9923. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CAP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9923)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CAP1 recombinant protein (Position: A2-R123). Human CAP1 shares 92.6% and 93.4% amino acid (aa) sequence identity with mouse and rat CAP1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9923 is reactive to CAP1 in Human, Mouse, Rat

Applications

PB9923 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

51.901kDa

Background of CAP1

Adenylyl cyclase-associated protein 1 is an enzyme that in humans is encoded by the CAP1 gene. This gene is mapped to 1p34.2. The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. And the human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CAP1 using anti-CAP1 antibody (PB9923).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: SMMC7721 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAP1 antigen affinity purified polyclonal antibody (Catalog # PB9923) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAP1 at approximately 60 kDa. The expected band size for CAP1 is at 52 kDa.

Gene/Protein Information For CAP1 (Source: Uniprot.org, NCBI)

Alternative Names

adenylyl cyclase-associated protein 1; CAP, adenylate cyclase-associated protein 1 (yeast); CAP1; CAP1-PEN; CAPCAP 1; SSKAP55 CAP1 CAP-PEN, CAP1 cyclase associated actin cytoskeleton regulatory protein 1 adenylyl cyclase-associated protein 1|CAP, adenylate cyclase-associated protein 1|adenylate cyclase associated protein 1|testis secretory sperm-binding protein Li 218p

For more info on CAP1, check out the CAP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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