Product Info Summary
SKU: | PA2103 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-12/CASP12 Antibody
View all Caspase-12 Antibodies
SKU/Catalog Number
PA2103
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-12/CASP12 Antibody catalog # PA2103. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-12/CASP12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2103)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2103 is reactive to Casp12 in Human, Rat
Applications
PA2103 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
47.854kDa
Background of Caspase-12
Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The CASP12 gene was mapped to chromosome 11q22.3. Csp12L attenuates the inflammatory and innate immune response to endotoxins and in doing so may constitute a risk factor for developing sepsis. The estrogen-mediated inhibition of CSP12L expression is a built-in mechanism that has evolved to protect females from infection by favoring a more robust inflammatory response to pathogens, perhaps notably in childbirth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-Caspase-12 antibody, PA2103, Western blotting
All lanes: Anti Caspase-12 (PA2103) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
Click image to see more details
Anti-Caspase-12 antibody, PA2103, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Click image to see more details
Anti-Caspase-12 antibody, PA2103, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For Casp12 (Source: Uniprot.org, NCBI)
Gene Name
Casp12
Full Name
Caspase-12
Weight
47.854kDa
Superfamily
peptidase C14A family
Alternative Names
Apoptosis Related Cysteine Protease; CASP 12; CASP12; CASP12P1; Caspase 12 pseudogene 1; Caspase12; Caspase-12; UNQ9415 Casp12|caspase 12|caspase-12|CASP-12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Casp12, check out the Casp12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Casp12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-12/CASP12 Antibody (PA2103)
Hello CJ!
PA2103 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Bone marrow stromal cells improved functional recovery in spinal cord injury rats partly via the Toll‑like receptor‑4/nuclear factor‑κB signaling pathway Shi Bai, Hao Zhou, and Lijuan Wu Exp Ther Med. 2019 Jan; 17(1): 444–448. Published online 2018 Oct
Zhao S, Xiong Z, Mao X, Meng D, Lei Q, Li Y, Deng P, Chen M, Tu M, Lu X, Yang G, He G. Plos One. 2013 Aug 27;8(8):E73665. Doi: 10.1371/Journal.Pone.0073665. Ecollection 2013. Atmospheric Pressure Room Temperature Plasma Jets Facilitate Oxidative A...
Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-Caspase-12/CASP12 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Caspase-12/CASP12 antibody PA2103. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-28
Question
Would anti-Caspase-12/CASP12 antibody PA2103 work for WB with lung?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
According to the expression profile of lung, CASP12 is highly expressed in lung. So, it is likely that anti-Caspase-12/CASP12 antibody PA2103 will work for WB with lung.
Boster Scientific Support
Answered: 2020-04-21
Question
Does anti-Caspase-12/CASP12 antibody PA2103 work on pig WB with right lung?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
Our lab technicians have not tested anti-Caspase-12/CASP12 antibody PA2103 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Caspase-12/CASP12 antibody PA2103 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig right lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-03
Question
Is a blocking peptide available for product anti-Caspase-12/CASP12 antibody (PA2103)?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do provide the blocking peptide for product anti-Caspase-12/CASP12 antibody (PA2103). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-17
Question
We are currently using anti-Caspase-12/CASP12 antibody PA2103 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
O. Bhatt
Verified customer
Asked: 2020-01-03
Answer
The anti-Caspase-12/CASP12 antibody (PA2103) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-03
Question
I was wanting to use your anti-Caspase-12/CASP12 antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
As indicated on the product datasheet, PA2103 anti-Caspase-12/CASP12 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
Would PA2103 anti-Caspase-12/CASP12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
As indicated on the product datasheet, PA2103 anti-Caspase-12/CASP12 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-18