Anti-Cathepsin D Ctsd Monoclonal Antibody

Boster Bio Anti-Cathepsin D Ctsd Monoclonal Antibody catalog # MA1013. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: MA1013
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC, WB

Product Name

Anti-Cathepsin D Ctsd Monoclonal Antibody

See all Cathepsin D products

SKU/Catalog Number

MA1013

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cathepsin D Ctsd Monoclonal Antibody catalog # MA1013. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cathepsin D Ctsd Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1013)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

CTD-19

Isotype

Mouse IgG2a

Immunogen

Human liver cathepsin D.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1013 is reactive to Ctsd in Human

Applications

MA1013 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat
Western blot, 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Ctsd (Source: Uniprot.Org, NCBI)

Uniprot ID

P24268

Gene ID

171293

Gene Name

Ctsd

Full Name

Cathepsin D

Weight

44.681kDa

Superfamily

peptidase A1 family

Alternative Names

cathepsin D (lysosomal aspartyl protease); Cathepsin D; CPSD; CTSD; EC 3.4.23; EC 3.4.23.5; lysosomal aspartyl peptidase; lysosomal aspartyl protease; MGC2311; neuronal 10 Ctsd|cathepsin D|cathepsin D

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ctsd, check out the Ctsd Infographic

Ctsd infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ctsd: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1013 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage checkpoint

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17 Customer Q&As for Anti-Cathepsin D Ctsd Monoclonal Antibody

Question

I see that the anti-Cathepsin D Monoclonal antibody MA1013 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-21

Question

Is there a BSA free version of anti-Cathepsin D Monoclonal antibody MA1013 available?

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin D Monoclonal antibody MA1013 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-07

Question

Will MA1013 anti-Cathepsin D Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

You can see on the product datasheet, MA1013 anti-Cathepsin D Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-12

Question

I have a question about product MA1013, anti-Cathepsin D Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-12

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1013 anti-Cathepsin D Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-12

Question

Does anti-Cathepsin D Monoclonal antibody MA1013 work on goat WB with melanoma?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

Our lab technicians have not tested anti-Cathepsin D Monoclonal antibody MA1013 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Cathepsin D Monoclonal antibody MA1013 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat melanoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-19

Question

I was wanting to use your anti-Cathepsin D Monoclonal antibody for IHC for human melanoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human melanoma identification?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

As indicated on the product datasheet, MA1013 anti-Cathepsin D Monoclonal antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human melanoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-26

Question

Is a blocking peptide available for product anti-Cathepsin D Monoclonal antibody (MA1013)?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

We do provide the blocking peptide for product anti-Cathepsin D Monoclonal antibody (MA1013). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-27

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-Cathepsin D Monoclonal antibody MA1013. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-22

Question

See below the WB image, lot number and protocol we used for melanoma using anti-Cathepsin D Monoclonal antibody MA1013. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-03

Question

We are interested in using your anti-Cathepsin D Monoclonal antibody for response to nutrient levels studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

Thanks for your inquiry. This MA1013 anti-Cathepsin D Monoclonal antibody is validated on human placenta tissue, intestinal cancer tissue, tissue lysate, lung cancer tissue, mammary cancer tissue, a549 whole cell lysate, hela whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-24

Question

Is this MA1013 anti-Cathepsin D Monoclonal antibody reactive to the isotypes of CTSD?

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

The immunogen of MA1013 anti-Cathepsin D Monoclonal antibody is Human liver cathepsin D. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-03-28

Question

We have observed staining in human kidney. What should we do? Is anti-Cathepsin D Monoclonal antibody supposed to stain kidney positively?

Verified Customer

Verified customer

Asked: 2018-03-07

Answer

From what I have seen in literature kidney does express CTSD. From what I have seen in Uniprot.org, CTSD is expressed in adult mammalian kidney, kidney, liver, melanoma, plasma, platelet, spleen, among other tissues. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789

Boster Scientific Support

Answered: 2018-03-07

Question

My team were content with the WB result of your anti-Cathepsin D Monoclonal antibody. However we have seen positive staining in adult mammalian kidney lysosome. melanosome. secreted, using this antibody. Is that expected? Could you tell me where is CTSD supposed to be expressed?

S. Lewis

Verified customer

Asked: 2017-11-03

Answer

From what I have seen in literature, adult mammalian kidney does express CTSD. Generally CTSD expresses in lysosome. melanosome. secreted,. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789

Boster Scientific Support

Answered: 2017-11-03

Question

Does anti-Cathepsin D Monoclonal antibody MA1013 work for IHC with melanoma?

Verified Customer

Verified customer

Asked: 2017-09-01

Answer

According to the expression profile of melanoma, CTSD is highly expressed in melanoma. So, it is likely that anti-Cathepsin D Monoclonal antibody MA1013 will work for IHC with melanoma.

Boster Scientific Support

Answered: 2017-09-01

Question

I was wanting to use to test anti-Cathepsin D Monoclonal antibody MA1013 on human melanoma for research purposes, then I may be interested in using anti-Cathepsin D Monoclonal antibody MA1013 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Krishna

Verified customer

Asked: 2015-10-16

Answer

The products we sell, including anti-Cathepsin D Monoclonal antibody MA1013, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-10-16

Question

We are currently using anti-Cathepsin D Monoclonal antibody MA1013 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

R. Brown

Verified customer

Asked: 2015-07-06

Answer

The anti-Cathepsin D Monoclonal antibody (MA1013) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-07-06

Question

We ordered your anti-Cathepsin D Monoclonal antibody for IHC on kidney in the past. I am using human, and We want to use the antibody for WB next. We are interested in examining kidney as well as platelet in our next experiment. Could give a recommendation on which antibody would work the best for WB?

K. Zhao

Verified customer

Asked: 2013-09-24

Answer

I have checked the website and datasheets of our anti-Cathepsin D Monoclonal antibody and I see that MA1013 has been validated on human in both IHC and WB. Thus MA1013 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-09-24

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MA1013
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