Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247

CD31/PECAM-1 antibody

Boster Bio Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 (Catalog # M01513-3). Tested in IHC, WB applications. This antibody reacts with Human. Cited in 9 publication(s).

Product Info Summary

SKU: M01513-3
Size: 100ul
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247

View all CD31/PECAM-1 Antibodies

SKU/Catalog Number

M01513-3

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 (Catalog # M01513-3). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01513-3)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM247

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the cytoplasmic domain of human CD31

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human CD31 (Platelet endothelial cell adhesion molecule, PECAM-1).

Reactive Species

M01513-3 is reactive to PECAM1 in Human

Applications

M01513-3 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

82.522kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:1000-1:2500 dilution
WB: 1:1000-1:2000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For PECAM1 (Source: Uniprot.org, NCBI)

Gene Name

PECAM1

Full Name

Platelet endothelial cell adhesion molecule

Weight

82.522kDa

Alternative Names

adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule PECAM1 CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM platelet and endothelial cell adhesion molecule 1 platelet endothelial cell adhesion molecule|CD31 antigen|platelet endothelial cell adhesion molecule-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PECAM1, check out the PECAM1 Infographic

PECAM1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PECAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01513-3 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The impact of vascular endothelial growth factor-transfected human endothelial cells on endothelialization and restenosis of stainless steel stents

EPCR promotes MGC803 human gastric cancer cell tumor angiogenesis in vitro through activating ERK1/2 and AKT in a PAR1%u2011dependent manner

The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex

Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling

Chen L, Chan Th, Yuan Yf, Hu L, Huang J, Ma S, Wang J, Dong Ss, Tang Kh, Xie D, Li Y, Guan Xy. J Clin Invest. 2010 Apr;120(4):1178-91. Doi: 10.1172/Jci40665. Epub 2010 Mar 24. Chd1L Promotes Hepatocellular Carcinoma Progression And Metastasis In M...

Qiu H, Yang B, Pei Zc, Zhang Z, Ding K. J Biol Chem. 2010 Oct 15;285(42):32638-46. Doi: 10.1074/Jbc.M110.105544. Epub 2010 Aug 2. Wss25 Inhibits Growth Of Xenografted Hepatocellular Cancer Cells In Nude Mice By Disrupting Angiogenesis Via Blocking...

Zhou S, Yi T, Liu R, Bian C, Qi X, He X, Wang K, Li J, Zhao X, Huang C, Wei Y. Mol Cell Proteomics. 2012 Jul;11(7):M112.017988. Doi: 10.1074/Mcp.M112.017988. Epub 2012 Apr 9. Proteomics Identification Of Annexin A2 As A Key Mediator In The Metasta...

Xiu J, Fan J, Li J, Cui G, Lei W. Biomed Res Int. 2014;2014:785146. Doi: 10.1155/2014/785146. Epub 2014 Jun 10. Different Angiogenic Abilities Of Self-Setting Calcium Phosphate Cement Scaffolds Consisting Of Different Proportions Of Fibrin Glue.

Wu Q, Chang Y, Zhang L, Zhang Y, Tian T, Feng G, Zhou S, Zheng Q, Han F, Huang F. J Cancer. 2013 Nov 21;4(9):727-35. Doi: 10.7150/Jca.7576. Ecollection 2013. Srpk1 Dissimilarly Impacts On The Growth, Metastasis, Chemosensitivity And Angiogenesis O...

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5 Customer Q&As for Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247

Question

We are currently using anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 M01513-3 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

The anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 (M01513-3) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-30

Question

We bought anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

I looked at the website and datasheets of our anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2019-11-28

Question

We have observed staining in human leukemic t-cell. What should we do? Is anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 supposed to stain leukemic t-cell positively?

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

From literature leukemic t-cell does express PECAM1. From Uniprot.org, PECAM1 is expressed in tendon of biceps brachii, lung, brain, platelet, liver, leukemic t-cell, among other tissues. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2018-10-03

Question

We are interested in using your anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 for rho protein signal transduction studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-09-07

Answer

Thanks for your inquiry. This M01513-3 anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone#RM247 is validated on jurkat cell lysate, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-09-07

Question

Our lab were satisfied with the WB result of your anti-CD31 (PECAM-1) Rabbit Monoclonal antibody, Clone

C. Krishna

Verified customer

Asked: 2017-08-01

Answer

From what I have seen in literature, liver does express PECAM1. Generally PECAM1 expresses in cell membrane. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087

Boster Scientific Support

Answered: 2017-08-01

$431
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Lead time for this item is typically 1-2 weeks

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M01513-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$431.00

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