radio
SKU PA1021-2
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Ig Isotype N/A
Applications IHC, ICC, WB

Overview

Product Name Anti-CD44 Antibody
SKU/Catalog Number PA1021-2
Storage & Handling [storage]
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for CD44 antigen(CD44) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Cite This Product Anti-CD44 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1021-2)
Immunogen [immunogen]
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Images And Assay Conditions

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Anti-CD44 antibody, PA1021-2, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

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Anti-CD44 antibody, PA1021-2, IHC(P)
IHC(P): Rat Intestine Tissue

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Anti-CD44 antibody, PA1021-2, ICC
ICC: HCT116 Cell

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Anti-CD44 antibody, PA1021-2, IHC(F)
IHC(F): Rat Intestine Tissue

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Anti-CD44 antibody, PA1021-2, Western blotting
All lanes: Anti CD44 (PA1021-2) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 82KD
Observed bind size: 82KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P16070
Gene Name CD44
Protein Name CD44 antigen
Tissue Specificity Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Alternative Names CD44 antigen;CDw44;Epican;Extracellular matrix receptor III;ECMR-III;GP90 lymphocyte homing/adhesion receptor;HUTCH-I;Heparan sulfate proteoglycan;Hermes antigen;Hyaluronate receptor;Phagocytic glycoprotein 1;PGP-1;Phagocytic glycoprotein I;PGP-I;CD44;CD44;LHR, MDU2, MDU3, MIC4;
Subcellular Localization Cell membrane; Single-pass type I membrane protein. Colocalizes with actin in membrane protrusions at wounding edges. .
Molecular Weight 81538 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. .
Background The CD44 gene, which is a transmembrane protein, is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. The gene, which contains 19 exons spanning some 50 kb of genomic DNA, is a widely expressed integral membrane protein that acts as a receptor for hyaluronan(HA) and is important to cell-extracellular matrix interaction. CD44 binding with HA can play an important role in cellular aggregation and tumor cell growth. CD44 is necessary for limb development and functions in a novel growth factor presentation mechanism. A specific CD44 splice variant is crucial for the proliferation of these mesenchymal cells. CD44 glycoproteins are involved in leukocyte extravasation but also in the regulation of growth factor activation, stability, and signaling. Moreover, it plays a pivotal role in arteriogenesis.

Order Product (PA1021-2)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
30ug sample size $99
100ug $280
100μg/vial $280
100ug+Free Biotin Secondary BA1054 $280
100μg/vial+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280
100μg/vial+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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We promise all of our products perform as described in datasheets.

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Publications

Lentivirus-mediated PLC?1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma
FoliumSennaeprotects against hydroxyl radical-induced DNA damage via antioxidant mechanism: anin vitrostudy
Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth
CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Overexpression of transcription factor Foxa2 and Hnf1? induced rat bone mesenchymal stem cells into hepatocytes
An effective inducer of dopaminergic neuron-like differentiation
Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes
Genistein Promotion of Osteogenic Differentiation through BMP2/SMAD5/RUNX2 Signaling
Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells
Xue L, Liang H, Jiang X. Mol Med Rep. 2011 Nov-Dec;4(6):1233-7. Doi: 10.3892/Mmr.2011.552. Epub 2011 Aug 12. Up-Regulation Of Cd44 Expression By Interleukin-13 In A Murine Model Of Asthma.
Han M, Liu M, Wang Y, Mo Z, Bi X, Liu Z, Fan Y, Chen X, Wu C. Mol Cell Biochem. 2012 Apr;363(1-2):427-36. Doi: 10.1007/S11010-011-1195-5. Epub 2011 Dec 21. Re-Expression Of Mir-21 Contributes To Migration And Invasion By Inducing Epithelial-Mesenc...
Cui G, Qi Z, Guo X, Qin J, Gui Y, Cai Z. Cell Biol Int. 2009 Mar;33(3):434-41. Rat Bone Marrow Derived Mesenchymal Progenitor Cells Support Mouse Es Cell Growth And Germ-Like Cell Differentiation.
Hefei W, Yu R, Haiqing W, Xiao W, Jingyuan W, Dongjun L. Cell Biol Int. 2015 Mar;39(3):253-63. Doi: 10.1002/Cbin.10387. Epub 2015 Jan 26. Morphological Characteristics And Identification Of Islet-Like Cells Derived From Rat Adipose-Derived Stem Ce...
Hou Y, Zou Q, Ge R, Shen F, Wang Y. Cell Res. 2012 Jan;22(1):259-72. Doi: 10.1038/Cr.2011.139. Epub 2011 Aug 23. The Critical Role Of Cd133(+)Cd44(+/High) Tumor Cells In Hematogenous Metastasis Of Liver Cancers.
Shi S, Han L, Deng L, Zhang Y, Shen H, Gong T, Zhang Z, Sun X. J Control Release. 2014 Nov 28;194:228-37. Doi: 10.1016/J.Jconrel.2014.09.005. Epub 2014 Sep 16. Dual Drugs (Microrna-34A And Paclitaxel)-Loaded Functional Solid Lipid Nanoparticles Fo...
Huang Y, Chang T, Yang C, Wu M. J Huazhong Univ Sci Technolog Med Sci. 2012 Aug;32(4):620-5. Doi: 10.1007/S11596-012-1007-5. Epub 2012 Aug 11. Mineralized And Osteoid Tissue From Dental Pulp Stem Cells On Micro-Arc Oxidation Titanium In Vitro.
Han M, Liu M, Wang Y, Chen X, Xu J, Sun Y, Zhao L, Qu H, Fan Y, Wu C. Plos One. 2012;7(6):E39520. Doi: 10.1371/Journal.Pone.0039520. Epub 2012 Jun 25. Antagonism Of Mir-21 Reverses Epithelial-Mesenchymal Transition And Cancer Stem Cell Phenotype T...
Chen Df, Zhang Hl, Du Sh, Li H, Zhou Jh, Li Yw, Zeng Hp, Hua Zc. Steroids. 2010 Dec 12;75(13-14):1119-26. Doi: 10.1016/J.Steroids.2010.07.009. Epub 2010 Jul 29. Cholesterol Myristate Suppresses The Apoptosis Of Mesenchymal Stem Cells Via Upregulat...
Dai K, Chen R, Ding Y, Niu Z, Fan J, Xu C. Cells Tissues Organs. 2014;200(2):132-40. Doi: 10.1159/000380762. Epub 2015 Apr 16. Induction Of Functional Hepatocyte-Like Cells By Overexpression Of Foxa3 And Hnf4?? In Rat Bone Marrow Mesenchymal Stem C...
Li X, Huang Y, Zheng L, Liu H, Niu X, Huang J, Zhao F, Fan Y. J Biomed Mater Res A. 2014 Apr;102(4):1092-101. Doi: 10.1002/Jbm.A.34774. Epub 2013 Jun 11. Effect Of Substrate Stiffness On The Functions Of Rat Bone Marrow And Adipose Tissue Derived ...
Dai J, Li Y, Zhou H, Chen J, Chen M, Xiao Z. Int J Biol Sci. 2013 Nov 21;9(10):1089-98. Doi: 10.7150/Ijbs.7367. Ecollection 2013. Genistein Promotion Of Osteogenic Differentiation Through Bmp2/Smad5/Runx2 Signaling.
Li Hm, Liu L, Mei X, Chen H, Liu Z, Zhao X. Life Sci. 2014 Jun 13;106(1-2):50-7. Doi: 10.1016/J.Lfs.2014.04.020. Epub 2014 Apr 26. Overexpression Of Inducible Nitric Oxide Synthase Impairs The Survival Of Bone Marrow Stem Cells Transplanted Into R...
Zhang G, Lan X, Yen Tc, Chen Q, Pei Z, Qin C, Zhang Y. Nucl Med Biol. 2012 Nov;39(8):1243-50. Doi: 10.1016/J.Nucmedbio.2012.06.010. Epub 2012 Jul 15. Therapeutic Gene Expression In Transduced Mesenchymal Stem Cells Can Be Monitored Using A Reporte...
Huang Xf, Yuan Sj, Yang C. Mol Med Rep. 2012 Sep;6(3):547-52. Doi: 10.3892/Mmr.2012.974. Epub 2012 Jun 29. Effects Of Total Flavonoids From Drynaria??fortunei On The Proliferation And Osteogenic Differentiation Of Rat Dental Pulp Stem C...
Du G, Liu Y, Dang M, Zhu G, Su R, Fan Y, Tan Z, Wang Lx, Fang J. Acta Histochem. 2014 Jul;116(6):1075-84. Doi: 10.1016/J.Acthis.2014.05.002. Epub 2014 Jun 21. Comparison Of Administration Routes For Adipose-Derived Stem Cells In The Treatment Of M...
Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd44 antibody, cd 44 antibody