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Product Info Summary
| SKU: | A00168-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Complement C3 C3 Antibody
SKU/Catalog Number
A00168-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Complement C3 C3 Antibody catalog # A00168-1. Tested in WB,ICC/IF,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Complement C3 C3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00168-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human p63 around the phosphorylation site of S455.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00168-1 is reactive to C3 in Human
Applications
A00168-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
187.148kDa
Background of C3
Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
FC: 1:50-1:100
ICC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of C3 using Anti-Complement C3 C3 Antibody (A00168-1).
ICC staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Complement C3 C3 Antibody A00168-1
Western blot analysis of C3 on different lysates using anti-C3 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For C3 (Source: Uniprot.org, NCBI)
Gene Name
C3
Full Name
Complement C3
Weight
187.148kDa
Alternative Names
AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; complement C3; complement component 3; complement component C3; CPAMD1acylation-stimulating protein cleavage product C3 AHUS5, ARMD9, ASPa, C3b, CPAMD1, HEL-S-62p, C3 complement C3 complement C3|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1|C3a anaphylatoxin|acylation-stimulating protein cleavage product|complement component 3|complement component C3a|complement component C3b|epididymis secretory sperm binding protein Li 62p|prepro-C3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on C3, check out the C3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for C3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Complement C3 C3 Antibody (A00168-1)
Hello CJ!
A00168-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Knockout of beta-2 microglobulin enhances cardiac repair by modulating exosome imprinting and inhibiting stem cell-induced immune rejection
Zhang Y,Wang Y,Shao L,Pan X,Liang C,Liu B,Zhang Y,Xie W,Yan B,Liu F,Yu XY,Li Y.Knockout of beta-2 microglobulin reduces stem cell-induced immune rejection and enhances ischaemic hindlimb repair via exosome/miR-24/Bim pathway.J Cell Mol Med.2020 Jan;24(1):
Species: Human,Mouse
A00168-1 usage in article: APP:IF, SAMPLE:GASTROCNEMIUS MUSCLE CELL, DILUTION:1:100
Shao L,Zhang Y,Pan X,Liu B,Liang C,Zhang Y,Wang Y,Yan B,Xie W,Sun Y,Shen Z,Yu XY,Li Y.Knockout of beta-2 microglobulin enhances cardiac repair by modulating exosome imprinting and inhibiting stem cell-induced immune rejection.Cell Mol Life Sci.2020 Mar;77
Species: Human,Rat
A00168-1 usage in article: APP:IF, SAMPLE:HEART TISSUE, DILUTION:1:100
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