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Product Info Summary
| SKU: | A04245-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Coronin 1a/TACO/CORO1A Antibody Picoband™
View all Coronin-1a Antibodies
SKU/Catalog Number
A04245-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Coronin 1a/TACO/CORO1A Antibody Picoband™ catalog # A04245-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Coronin 1a/TACO/CORO1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04245-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Coronin 1a/TACO/CORO1A recombinant protein (Position: E173-K461).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04245-2 is reactive to CORO1A in Human, Mouse, Rat
Applications
A04245-2 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
57 kDa
Calculated molecular weight
51.026kDa
Background of Coronin-1a
Coronin-1A is a protein that in humans is encoded by the CORO1A gene. This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coronin 1a/TACO/CORO1A antigen affinity purified polyclonal antibody (Catalog # A04245-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coronin 1a/TACO/CORO1A at approximately 57KD. The expected band size for Coronin 1a/TACO/CORO1A is at 57KD.
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Figure 10. Flow Cytometry analysis of THP-1 cells using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Overlay histogram showing THP-1 cells stained with A04245-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 2. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Coronin 1a/TACO/CORO1A was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 11. Flow Cytometry analysis of U937 cells using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2).
Overlay histogram showing U937 cells stained with A04245-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (A04245-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CORO1A (Source: Uniprot.org, NCBI)
Gene Name
CORO1A
Full Name
Coronin-1A
Weight
51.026kDa
Superfamily
WD repeat coronin family
Alternative Names
CLIPINA; clipin-A; CORO1; coronin, actin binding protein, 1A; coronin, actin-binding protein, 1A; coronin-1; coronin-1A; Coronin-like protein A; Coronin-like protein p57; FLJ41407; HCORO1; MGC117380; p57; TACOCLABP; Tryptophan aspartate-containing coat protein CORO1A CLABP, CLIPINA, HCORO1, IMD8, TACO, p57 coronin 1A coronin-1A|clipin-A|coronin, actin binding protein, 1A|coronin-1|coronin-like protein A|coronin-like protein p57|tryptophan aspartate-containing coat protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CORO1A, check out the CORO1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CORO1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Coronin 1a/TACO/CORO1A Antibody Picoband™ (A04245-2)
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