Anti-CXCR4 Monoclonal Antibody

Product Name

Anti-CXCR4 Monoclonal Antibody

View all CXCR4 Antibodies

SKU/Catalog Number

M00031

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CXCR4 Monoclonal Antibody catalog # M00031. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CXCR4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00031)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CID-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CXCR4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00031 is reactive to CXCR4 in Human, Mouse

Applications

M00031 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

131 kDa

Calculated molecular weight

39.746kDa

Background of CXCR4

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:100-1:200
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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Western blot analysis of CXCR4 expression in Jurkat cell lysate.

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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, using CXCR4 Antibody.

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Immunofluorescent analysis of Jurkat cells, using CXCR4 Antibody .

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For CXCR4 (Source: Uniprot.org, NCBI)

Alternative Names

CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS C-X-C motif chemokine receptor 4 C-X-C chemokine receptor type 4|CD184 antigen|LPS-associated protein 3|SDF-1 receptor|chemokine (C-X-C motif) receptor 4|fusin|leukocyte-derived seven transmembrane domain receptor|lipopolysaccharide-associated protein 3|neuropeptide Y receptor Y3|neuropeptide Y3 receptor|seven transmembrane helix receptor|seven-transmembrane-segment receptor, spleen|stromal cell-derived factor 1 receptor

For more info on CXCR4, check out the CXCR4 Infographic

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In this infographic, you will see the following information for CXCR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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