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Product Info Summary
| SKU: | M00745-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00745-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody catalog # M00745-3. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00745-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CIC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin B1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00745-3 is reactive to CCNB1 in Human
Applications
M00745-3 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
133 kDa
Calculated molecular weight
48.337kDa
Background of Cyclin B1
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:30
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Western blot analysis of Cyclin B1 expression in (1)HaCaT cell lysates; (2)HepG2 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cyclin B1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CCNB1 (Source: Uniprot.org, NCBI)
Gene Name
CCNB1
Full Name
G2/mitotic-specific cyclin-B1
Weight
48.337kDa
Superfamily
cyclin family
Alternative Names
CCNB; CCNB1; Cyclin B1; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1 CCNB1 CCNB cyclin B1 G2/mitotic-specific cyclin-B1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNB1, check out the CCNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody (M00745-3)
Hello CJ!
M00745-3 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang H,Su Y,Wang J,Gao Y,Yang F,Li G,Shi Q.Ginsenoside Rb1 promotes the growth of mink hair follicle via PI3K/AKT/GSK-3β signaling pathway.Life Sci.2019 Jul 15;229:210-218.doi:10.1016/j.lfs.2019.05.033.Epub 2019 May 16.PMID:31102746.
Species: American Minks
M00745-3 usage in article: APP:WB, SAMPLE:HAIR FOLLICLES AND DPCS, DILUTION:1:400
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody
Question
Is this M00745-3 anti-Cyclin B1 Rabbit Monoclonal antibody reactive to the isotypes of CCNB1?
Verified Customer
Verified customer
Asked: 2018-11-13
Answer
The immunogen of M00745-3 anti-Cyclin B1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Cyclin B1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-13
Question
I was wanting to use your anti-Cyclin B1 Rabbit Monoclonal antibody for IP for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
It shows on the product datasheet, M00745-3 anti-Cyclin B1 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-04
Question
We are interested in to test anti-Cyclin B1 Rabbit Monoclonal antibody M00745-3 on human cervix carcinoma for research purposes, then I may be interested in using anti-Cyclin B1 Rabbit Monoclonal antibody M00745-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Edwards
Verified customer
Asked: 2015-09-18
Answer
The products we sell, including anti-Cyclin B1 Rabbit Monoclonal antibody M00745-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-09-18
