Product Info Summary
SKU: | PA1245-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Cyclin D1/CCND1 Antibody
SKU/Catalog Number
PA1245-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin D1/CCND1 Antibody catalog # PA1245-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin D1/CCND1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1245-2)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Cyclin D1, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1245-2 is reactive to CCND1 in Human, Rat
Applications
PA1245-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
33 kDa
Calculated molecular weight
33.729kDa
Background of CCND1
Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL; 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (PA1245-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis Tissue Lysate,
Lane 2: Human Placenta Tissue Lysate,
Lane 3: Rat Brain Tissue Lysate,
Lane 4: MCF-7 Whole Cell Lysate,
Lane 5: COLO320 Whole Cell Lysate,
Lane 6: SW620 Whole Cell Lysate,
Lane 7: MM231 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified polyclonal antibody (Catalog # PA1245-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 33KD. The expected band size for Cyclin D1 is at 33KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of U-87MG cells using anti-Cyclin D1 antibody (PA1245-2).
Overlay histogram showing U-87MG cells stained with PA1245-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin D1 Antibody (PA1245-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CCND1 (Source: Uniprot.org, NCBI)
Gene Name
CCND1
Full Name
G1/S-specific cyclin-D1
Weight
33.729kDa
Superfamily
cyclin family
Alternative Names
B-cell lymphoma 1 protein; BCL-1 oncogene; Bcl-1; BCL1D11S287E; CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); Cyclin D1; Cyl-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; PRAD1; PRAD1B-cell CLL/lymphoma 1; U21B31 CCND1 BCL1, D11S287E, PRAD1, U21B31 cyclin D1 G1/S-specific cyclin-D1|B-cell CLL/lymphoma 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCND1, check out the CCND1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin D1/CCND1 Antibody (PA1245-2)
Hello CJ!
PA1245-2 has been cited in 45 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu S,Sun Y,Zhao R,Wang Y,Zhang W,Pang W. Isoleucine increases muscle mass through promoting myogenesis and intramyocellular fat deposition. Food Funct.2021 Jan 7;12(1):144-153.doi: 10.1039/d0fo02156c.Epub 2020 Dec 8. PMID:33289736.
Species: Mouse
PA1245-2 usage in article: APP:WB, SAMPLE:MUSCLE TISSUE AND C2C12 CELLS, DILUTION:1:500
Wang S,Zhang C,Zhang X.Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.Mol Med Rep.2020 Feb;21(2):589-596.doi:10.3892/mmr.2019.10887.Epub 2019 Dec 17.PMID:31974617; PMC
Species: Human
PA1245-2 usage in article: APP:WB, SAMPLE:HPASMCS, DILUTION:1:200
Wang C,Shao S,Deng L,Wang S,Zhang Y. LncRNA SNHG12 regulates the radiosensitivity of cervical cancer through the miR-148a/CDK1 pathway. Cancer Cell Int.2020 Dec 1;20(1):554.doi:10.1186/s12935-020-01654-5.PMID:33292254;PMCID:PMC7708190.
Species: Human
PA1245-2 usage in article: APP:WB, SAMPLE:SIHA AND HELA CELLS, DILUTION:1:2000
Effects of Buddleja officinalis granules on apoptosis factors Bax, Caspase-3, Fas, and FasL in lacrimal gland cells of castrated male rabbits Genyan Qin, et al. J Ophthalmol. 2019 Feb 5;2019:5916243. doi: 10.1155/2019/5916243. eCollection 2019.
Effects of xanthohumol-rich hop extract on the differentiation of preadipocytes
Overexpression of MYCT1 inhibits proliferation and induces apoptosis in human acute myeloid leukemia HL-60 and KG-1a cells in vitro and in vivo
Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3-E1 cells
LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFR? signaling
Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice
Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study
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