Anti-DARC ACKR1 Antibody

Product Name

Anti-DARC ACKR1 Antibody

View all DARC Antibodies

SKU/Catalog Number

A04540

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DARC ACKR1 Antibody (Catalog # A04540). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

DARC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DARC ACKR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04540)

Host

Rabbit

Contents

DARC Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DARC antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human DARC. The immunogen is located within the last 50 amino acids of DARC.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.

Reactive Species

A04540 is reactive to ACKR1 in Human, Mouse, Rat

Applications

A04540 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

35.553kDa

Background of DARC

DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DARC antibody can be used for detection of DARC by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of DARC in human cerebellum tissue lysate with DARC antibody at 1 μg/mL.

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Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 μg/mL.

Gene/Protein Information For ACKR1 (Source: Uniprot.org, NCBI)

Alternative Names

ACKR1; CCBP1; CD234 antigen; CD234; DARC; Dfy; Duffy Antigen; Duffy antigen/chemokine receptor; Duffy blood group antigen; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPD; GPDWBCQ1; GpFy; Plasmodium vivax receptor ACKR1 CCBP1, CD234, DARC, DARC/ACKR1, Dfy, FY, GPD, GpFy, WBCQ1 atypical chemokine receptor 1 (Duffy blood group) atypical chemokine receptor 1|Duffy antigen chemokine receptor|Duffy blood group antigen|Duffy blood group system protein|Duffy blood group, chemokine receptor|Fy glycoprotein|glycoprotein D|plasmodium vivax receptor

For more info on ACKR1, check out the ACKR1 Infographic

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In this infographic, you will see the following information for ACKR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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