Anti-DCLK1 Antibody

Product Name

Anti-DCLK1 Antibody

View all DCLK1 Antibodies

SKU/Catalog Number

A03089

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-DCLK1 Antibody (Catalog # A03089). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

DCLK1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-DCLK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03089)

Host

Rabbit

Contents

DCLK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

DCLK1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK1. The immunogen is located within the last 50 amino acids of DCLK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

PID antibody is predicted to not cross-react with MTA2

Reactive Species

A03089 is reactive to DCLK1 in Human, Mouse, Rat

Applications

A03089 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

82.224kDa

Background of DCLK1

DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DCLK1 antibody can be used for detection of DCLK1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of DCLK1 in human brain tissue lysate with DCLK1 antibody at (A) 0.5 and (B) 1 μg/mL.

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Immunohistochemistry of DLCK1 in rat brain tissue with DLCK1 antibody at 2.5 μg/mL.

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Immunofluorescence of DCLK1 in mouse brain tissue with DCLK1 Antibody at 20 μg/mL.

Green: DCLK1 Antibody (A03089)
Blue: DAPI staining

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Immunohistochemistry of DCLK1 in mouse brain tissue with DCLK1 Antibody at 5 μg/mL.

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Western blot analysis of DCLK1 expression in rat brain tissue lysate with DCLK1 antibody at (A) 1 and (B) 2 μg/ml.

Gene/Protein Information For DCLK1 (Source: Uniprot.org, NCBI)

Alternative Names

CL1; CLICK1; DCAMKL1; DCAMKL1CLICK1; DCDC3A; DCDC3ACL1; DCLK; DCLK1; doublecortin and CaM kinase-like 1; Doublecortin domain-containing protein 3A; doublecortin-like kinase 1Doublecortin-like and CAM kinase-like 1; EC 2.7.11; KIAA0369EC 2.7.11.1; serine/threonine-protein kinase DCLK1 DCLK1 CL1, CLICK1, DCAMKL1, DCDC3A, DCLK doublecortin like kinase 1 serine/threonine-protein kinase DCLK1|doublecortin domain-containing protein 3A|doublecortin-like and CAM kinase-like 1

For more info on DCLK1, check out the DCLK1 Infographic

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In this infographic, you will see the following information for DCLK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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