Product Info Summary
SKU: | A01094-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-DNAM-1 CD226 Antibody
View all DNAM-1/CD226 Antibodies
SKU/Catalog Number
A01094-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DNAM-1 CD226 Antibody catalog # A01094-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNAM-1 CD226 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01094-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human DNAM-1 around the non-phosphorylation site of S329.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01094-1 is reactive to CD226 in Human, Monkey, Mouse
Applications
A01094-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38.614kDa
Background of DNAM-1/CD226
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.
Gluck SL, et al. (2004) J Clin Invest; 114(12): 1696-1699
Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9784-9789
Tian D, et al. (2002) Mol Cell Biol; 22(8): 2650-2662
Lu Z, et al. (2001) Mol Cell Biol; 21(12): 4016-4031
Krishnan HH, et al. (2006) J Virol; 80(3): 1167-1180
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-DNAM-1 CD226 Antibody A01094-1
Western Blot (WB) analysis of Mouse Brain cells using DNAM-1 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-DNAM-1 CD226 Antibody A01094-1
Western Blot (WB) analysis of COS7 cells using DNAM-1 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 3. Western blotting validation for Anti-DNAM-1 CD226 Antibody A01094-1
Western Blot (WB) analysis of AD293 using DNAM-1 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CD226 (Source: Uniprot.org, NCBI)
Gene Name
CD226
Full Name
CD226 antigen
Weight
38.614kDa
Alternative Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1 CD226 DNAM-1, DNAM1, PTA1, TLiSA1 CD226 molecule CD226 antigen|DNAX accessory molecule-1|T lineage-specific activation antigen 1 antigen|adhesion glycoprotein|platelet and T cell activation antigen 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD226, check out the CD226 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD226: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNAM-1 CD226 Antibody (A01094-1)
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No publications found for A01094-1
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