Product Info Summary
SKU: | M02276 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DUSP1/Mkp 1 Rabbit Monoclonal Antibody
View all MKP-1/DUSP1 Antibodies
SKU/Catalog Number
M02276
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-DUSP1/Mkp 1 Rabbit Monoclonal Antibody catalog # M02276. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DUSP1/Mkp 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02276)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IHB-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human DUSP1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02276 is reactive to DUSP1 in Human, Mouse, Rat
Applications
M02276 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
39.298kDa
Background of MKP-1/DUSP1
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of DUSP1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For DUSP1 (Source: Uniprot.org, NCBI)
Gene Name
DUSP1
Full Name
Dual specificity protein phosphatase 1
Weight
39.298kDa
Superfamily
protein-tyrosine phosphatase family
Alternative Names
CL100; CL100MAP kinase phosphatase 1; dual specificity phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP1; EC 3.1.3.16; EC 3.1.3.48; HVH1; Mitogen-activated protein kinase phosphatase 1; MKP1; MKP-1; MKP-1dual specificity protein phosphatase 1; Protein-tyrosine phosphatase CL100; PTPN10; PTPN10serine/threonine specific protein phosphatase; VH1 DUSP1 CL100, HVH1, MKP-1, MKP1, PTPN10 dual specificity phosphatase 1 dual specificity protein phosphatase 1|CL 100|MAP kinase phosphatase 1|dual specificity protein phosphatase hVH1|mitogen-activated protein kinase phosphatase 1|protein-tyrosine phosphatase CL100|serine/threonine specific protein phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DUSP1, check out the DUSP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DUSP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DUSP1/Mkp 1 Rabbit Monoclonal Antibody (M02276)
Hello CJ!
M02276 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
USP49 inhibits ischemia–reperfusion‐induced cell viability suppression and apoptosis in human AC16 cardiomyocytes through DUSP1–JNK1/2 signaling
Lin S,Li Q,Jiang S,Xu Z,Jiang Y,Liu L,Jiang J,Tong Y,Wang P.Crocetin ameliorates chronic restraint stress-induced depression-like behaviors in mice by regulating MEK/ERK pathways and gut microbiota. J Ethnopharmacol.2020 Nov 23:113608.doi:10.1016/j.jep.20
Species: Mouse
M02276 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50
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