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- Table of Contents
SLC1A3 antibody (PA2185) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of GLAST/SLC1A3 using anti-GLAST/SLC1A3 antibody (PA2185).
GLAST/SLC1A3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLAST/SLC1A3 Antibody (PA2185) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GLAST/SLC1A3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLAST/SLC1A3 Antibody (PA2185) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for SLC1A3? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the SLC1A3 Western Blot GuideBundle Save
Anti-EAAT1/SLC1A3 Antibody Picoband®
SLC1A3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
SLC1A3 antibody was positively detected by WB in:
human SH-SY5Y whole cell, rat brain tissu, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC1A3 antibody was positively detected by IHC in:
human brain tissue, ,mouse brain tissue, rat brain tissu
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Rat, Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-EAAT1/SLC1A3 Antibody Picoband®
View all SLC1A3 Antibodies
View all SLC1A3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1, different from the related rat and mouse sequences by three amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
59.6 kDa
Background of SLC1A3
Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. SLC1A3 is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). This gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. This gene acts as a symport by cotransporting sodium.
Cite This Product
Anti-EAAT1/SLC1A3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2185)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
89%
Moderate Homology
Rat
89%
Moderate Homology
Rabbit
89%
Moderate Homology
Dog
84%
Moderate Homology
Cow
68%
Low homology
Sheep
68%
Low homology
Zebrafish
68%
Low homology
Drosophila
21%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC1A3 antibody
Immunohistochemistry (IHC) protocol for SLC1A3 antibody
Publications
Application:
Species:
Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells
Published: 2023-05-30
Journal: iScience
Application: IF
Impact Factor: 5.80
Species: Human
PMID: 37534135
Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia
Published: 2021-09-01
Journal: Nature Communications
Application: IHC
Impact Factor: 14.92
Species: Human
PMID: 34471104
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Product Promise
We promise Anti-EAAT1/SLC1A3 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2185
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 19, 2026