Product Info Summary
SKU: | A07649 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-EID1 Antibody
SKU/Catalog Number
A07649
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-EID1 Antibody (Catalog # A07649). Tested in WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EID1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07649)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human EID1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07649 is reactive to EID1 in Human
Applications
A07649 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
20.876kDa
Background of EID1
Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms. UniProtKB Q9DCR4
MacLellan W.R., Mol. Cell. Biol. 20:8903-8915(2000).
Wen H., Ao S.; Gene 263:85-92(2001).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HeLa cells, using EID1 antibody A07649.
Click image to see more details
Immunofluorescence analysis of A549 cells, using EID1 antibody A07649.
Protein Target Info & Infographic
Gene/Protein Information For EID1 (Source: Uniprot.org, NCBI)
Gene Name
EID1
Full Name
EP300-interacting inhibitor of differentiation 1
Weight
20.876kDa
Alternative Names
21 kDa pRb-associated protein; C15orf3PTD014; CREBBP/EP300 inhibitor 1; CREBBP/EP300 inhibitory protein 1MGC138884; CRI1EP300-interacting inhibitor of differentiation 1; E1A-like inhibitor of differentiation 1; EID-1MGC138883; EP300 interacting inhibitor of differentiation 1; IRO45620; NB4 apoptosis related protein; Rb- and p300-binding protein EID-1; RBP21; retinoblastoma protein-associated protein EID1 C15orf3, CRI1, EID-1, IRO45620, PNAS-22, PTD014, RBP21 EP300 interacting inhibitor of differentiation 1 EP300-interacting inhibitor of differentiation 1|21 kDa pRb-associated protein|CREBBP/EP300 inhibitor 1|CREBBP/EP300 inhibitory protein 1|E1A-like inhibitor of differentiation 1|NB4 apoptosis related protein|Rb- and p300-binding protein EID-1|retinoblastoma protein-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EID1, check out the EID1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EID1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EID1 Antibody (A07649)
Hello CJ!
No publications found for A07649
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-EID1 Antibody
Question
We are currently using anti-EID1 antibody A07649 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-EID1 antibody (A07649) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
I have a question about product A07649, anti-EID1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07649 anti-EID1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-10
Question
Will A07649 anti-EID1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Miller
Verified customer
Asked: 2018-01-16
Answer
As indicated on the product datasheet, A07649 anti-EID1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-16
Question
Is there a BSA free version of anti-EID1 antibody A07649 available?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EID1 antibody A07649 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-01
Question
See attached the WB image, lot number and protocol we used for neonatal brain {eco:0000312 using anti-EID1 antibody A07649. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-25
Question
I was wanting to use your anti-EID1 antibody for WB for human neonatal brain eco:0000312 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been validated and is this antibody a good choice for human neonatal brain {eco:0000312 identification?
C. Jones
Verified customer
Asked: 2014-04-17
Answer
As indicated on the product datasheet, A07649 anti-EID1 antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human neonatal brain {eco:0000312 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-04-17