Anti-eNOS (Ab-1177) NOS3 Antibody

Product Name

Anti-eNOS (Ab-1177) NOS3 Antibody

View all eNOS Antibodies

SKU/Catalog Number

A01604-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-eNOS (Ab-1177) NOS3 Antibody (Catalog # A01604-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-eNOS (Ab-1177) NOS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01604-1)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Clone Number

3A1

Isotype

IgG

Immunogen

Peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01604-1 is reactive to NOS3 in Human, Mouse, Rat

Applications

A01604-1 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

133.275kDa

Background of eNOS

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 140kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NOS3.

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Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Ab-1177) Antibody #A01604-1.

Gene/Protein Information For NOS3 (Source: Uniprot.org, NCBI)

Alternative Names

Constitutive NOS; EC 1.14.13.39; ECNOS; Endothelial NOS; endothelial; eNOS; eNOScNOS; nitric oxide synthase 3 (endothelial cell); NOS type III; NOS3; NOSIII NOS3 ECNOS, eNOS nitric oxide synthase 3 nitric oxide synthase, endothelial|EC-NOS|NOS type III|NOSIII|cNOS|constitutive NOS|endothelial NOS|nitric oxide synthase 3 (endothelial cell)|nitric oxide synthase 3 transcript variant eNOS-delta20|nitric oxide synthase 3 transcript variant eNOS-delta20-21|nitric oxide synthase 3 transcript variant eNOS-delta21

For more info on NOS3, check out the NOS3 Infographic

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In this infographic, you will see the following information for NOS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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