Anti-ERAP1 (K467) Antibody

Product Name

Anti-ERAP1 (K467) Antibody

View all Aminopeptidase PILS/ARTS1 Antibodies

SKU/Catalog Number

A02021-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-ERAP1 (K467) Antibody catalog # A02021-2. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ERAP1 (K467) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02021-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ARTS-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02021-2 is reactive to ERAP1 in Human, Mouse, Rat

Applications

A02021-2 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

107.235kDa

Background of Aminopeptidase PILS/ARTS1

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.

Hattori A., J. Biochem. 130:235-241(2001).
Nakajima D., DNA Res. 9:99-106(2002).
Clark H.F., Genome Res. 13:2265-2270(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200

Validation Images & Assay Conditions

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Figure 2. Immunohistochemistry validation of ERAP1 using Anti-ERAP1 (K467) Antibody (A02021-2).

Immunohistochemistry (IHC) analyzes of ERAP1 (K467) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC

Click image to see more details

Figure 1. Western blotting validation for Anti-ERAP1 (K467) Antibody A02021-2

Western blot (WB) analysis of ERAP1 (K467) pAb at 1:500 dilution
Lane1:HepG2 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(20ug)
Lane3:PC12 whole cell lysate(20ug)
Lane4:AML-12 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For ERAP1 (Source: Uniprot.org, NCBI)

Alternative Names

Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator ERAP1 A-LAP, ALAP, APPILS, ARTS-1, ARTS1, ERAAP, ERAAP1, PILS-AP, PILSAP endoplasmic reticulum aminopeptidase 1 endoplasmic reticulum aminopeptidase 1|CTD-2260A17.2|adipocyte-derived leucine aminopeptidase|aminopeptidase PILS|aminopeptidase regulator of TNFR1 shedding|endoplasmic reticulum aminopeptidase associated with antigen processing|puromycin-insensitive leucyl-specific aminopeptidase|type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

For more info on ERAP1, check out the ERAP1 Infographic

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In this infographic, you will see the following information for ERAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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