Anti-ERK2 Monoclonal Antibody

Product Name

Anti-ERK2 Monoclonal Antibody

View all ERK2 Antibodies

SKU/Catalog Number

M00030

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ERK2 Monoclonal Antibody catalog # M00030. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ERK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00030)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

II-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ERK2 Act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00030 is reactive to MAPK1 in Human, Mouse, Rat

Applications

M00030 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

131 kDa

Calculated molecular weight

41.39kDa

Background of ERK2

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
FC 1:40

Validation Images & Assay Conditions

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Western blot analysis of ERK2 in (1) A431 cell lysate; (2) HeLa cell lysate.

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Human renal cancer, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Rat lung, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Mouse ovary, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Rat skin, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using ERK2 Antibody.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For MAPK1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.24; ERK; ERK2; ERK-2; ERK2MAP kinase isoform p42; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 1; MAPK1; MAPK2; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42mapk; p42-MAPK; PRKM1; PRKM1MAPK 2; PRKM2; protein tyrosine kinase ERK2 MAPK1 ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK mitogen-activated protein kinase 1 mitogen-activated protein kinase 1|MAP kinase 1|MAP kinase 2|MAPK 2|extracellular signal-regulated kinase 2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2

For more info on MAPK1, check out the MAPK1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAPK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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