Anti-FACL4 Rabbit Monoclonal Antibody

Product Name

Anti-FACL4 Rabbit Monoclonal Antibody

View all FACL4 Antibodies

SKU/Catalog Number

M04372-1

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-FACL4 Rabbit Monoclonal Antibody catalog # M04372-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FACL4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04372-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

26A48

Isotype

IgG

Immunogen

A synthesized peptide derived from FACL4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04372-1 is reactive to ACSL4 in Human, Mouse, Rat

Applications

M04372-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

79 kDa

Calculated molecular weight

79.188kDa

Background of FACL4

Plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FACL4 using anti-FACL4 antibody (M04372-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FACL4 antigen affinity purified monoclonal antibody (Catalog # M04372-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FACL4 at approximately 79 kDa. The expected band size for FACL4 is at 79 kDa.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

Gene/Protein Information For ACSL4 (Source: Uniprot.org, NCBI)

Alternative Names

ACS4mental retardation, X-linked 68; acyl-CoA synthetase 4; acyl-CoA synthetase long-chain family member 4; EC 6.2.1.3; FACL4long-chain 4; LACS 4; LACS4MRX68; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 4; long-chain fatty-acid-Coenzyme A ligase 4; long-chain-fatty-acid--CoA ligase 4; mental retardation, X-linked 63; MRX63 ACSL4 ACS4, FACL4, LACS4, MRX63, MRX68 acyl-CoA synthetase long chain family member 4 long-chain-fatty-acid--CoA ligase 4|acyl-CoA synthetase 4|arachidonate--CoA ligase|fatty-acid-Coenzyme A ligase, long-chain 4|lignoceroyl-CoA synthase|long-chain acyl-CoA synthetase 4|long-chain fatty-acid-Coenzyme A ligase 4

For more info on ACSL4, check out the ACSL4 Infographic

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In this infographic, you will see the following information for ACSL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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